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Periovax3, a Key Antigenic Determinant With Immunoprotective Potential Against Periodontal Pathogen Publisher Pubmed



Hashemi S1 ; Sepehrizadeh Z1 ; Setayesh N1 ; Kadkhoda Z2 ; Faramarzi MA1 ; Shahverdi AR1 ; Glogauer M3 ; Amin M4, 5
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Authors Affiliations
  1. 1. Department of Pharmaceutical Biotechnology, Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Periodontology, School of Dentistry, Tehran University of Medical Science, Tehran, Iran
  3. 3. Matrix Dynamics Group, Faculty of Dentistry, University of Toronto, Toronto, ON, Canada
  4. 4. Department of Drug and Food Control, Recombinant Vaccine Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. The Institute of Pharmaceutical Sciences, Tehran University of Medical Sciences, Tehran, Iran

Source: Microbial Pathogenesis Published:2019


Abstract

Treponema (T.) denticola is one of the key etiological agents in the development of periodontitis. The major outer sheath protein (Msp) of T. denticola has been shown to mediate pathogenesis and to facilitate adhesion of T. denticola to mucosal surfaces. This study aimed to find short polypeptides in the amino acid sequence of Msp which may be immunogenic and might elicit protective antisera against T. denticola. The complete msp sequence was divided into six fragments and the corresponding genes were cloned and expressed. Antisera against the polypeptides were raised in rabbits and fragment 3 (F3), hereinafter called PerioVax3 was the most potent fragment of the Msp in terms of yielding high titer antiserum. An adhesion assay was done to examine the inhibitory effects of antisera on the attachment of T. denticola to human gingival fibroblasts (HGFs) and human fibronectin. Antiserum against PerioVax3 significantly inhibited attachment of T. denticola to the substratum. Also, antiserum against PerioVax3 inhibited detachment of HGFs upon T. denticola exposure. To begin examining the clinical relevance of this work, blood samples from 12 sever periodontitis patients were collected and the sera were used in western blotting against the recombinant polypeptides. Periodontitis patient antisera exclusively detected PerioVax3 in western blotting. The data suggest that PerioVax3 carries epitopes that may trigger humoral immunity against T. denticola, which may protect against its adhesion functions. The complexity of periodontitis suggests that PerioVax3 may be considered for testing as a component of an experimental multivalent periodontal vaccine in further preclinical and clinical studies. © 2019 Elsevier Ltd
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