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Dual Targeting of Breast Cancer by Chitosan/Poly Lactic-Co-Glycolic Acid Nanodelivery Systems: Surface Activation With Folic Acid/Aptamers, and Co-Encapsulated With Sorafenib and Quercetin Publisher



Narmani A1 ; Ganji S2 ; Amirishoar M3 ; Hajikarimi F4 ; Mazandarani A5 ; Karimi M6 ; Mohammadinejad A7 ; Azadpour A8 ; Jahedi R9 ; Assadpour E10, 11 ; Jafari SM12, 13
Authors
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Authors Affiliations
  1. 1. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, 14177-55469, Tehran, Iran
  2. 2. Faculty of Medicine, Mashhad University of Medical Science, Mashhad, Iran
  3. 3. Department of Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  4. 4. Department of Applied Chemistry, Islamic Azad University, Tehran Pharmaceutical Branch, Tehran, Iran
  5. 5. Department of Molecular and Cell Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
  6. 6. Department of Biology, Faculty of Science and Research, Islamic Azad University, Tehran, Iran
  7. 7. Department of Chemistry, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran
  8. 8. Department of Biotechnology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
  9. 9. Department of Plant Biology, Faculty of Natural Sciences, University of Tabriz, 51666-16471, Tabriz, Iran
  10. 10. Food Industry Research Co., Gorgan, Iran
  11. 11. Food and Bio-Nanotech International Research Center (Fabiano), Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
  12. 12. Department of Food Materials and Process Design Engineering, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
  13. 13. Halal Research Center of IRI, Iran Food and Drug Administration, Ministry of Health and Medical Education, Tehran, Iran

Source: Carbohydrate Polymer Technologies and Applications Published:2025


Abstract

Breast cancer has a high rate of incidence and is one of the leading causes of death worldwide. Therefore, a breakthrough aptamer (Apt)- and folic acid (FA)-targeted chitosan (CS)-poly lactic-co-glycolic acid (PLGA) nanocarrier was developed for co-delivery of sorafenib (So) and quercetin (Qu) to MCF-7 and MDA-MB-231 breast cancer cells. Nanometric size (50 to 110 nm), semi-spherical and spherical shape, rough surface, and near-to-neutral surface charge (3.8 mV) were measured for Apt-PLGA-So-Qu-CS-FA nanocarriers. The So and Qu drugs content was obtained at about 2.85% and 11.63%, respectively. Controlled release (6.3% (So) and 7.2% (Qu) within 2 h) and pH-sensitive drug release was observed for this nanocarrier. MTT assay indicated lower cell viability for MCF-7 cells after treatment with Apt-PLGA-So-Qu-CS-FA nanocarriers (10% cell viability after 24 h treatment with 250 nm; IC50 = 100 nm). Caspase9 and P53 genes expression was increased by 11.8 and 12.8 folds while > 5 folds reduction was observed for Bcl2 expression after treatment with Apt-PLGA-So-Qu-CS-FA. Also, this nanocarrier led to > 90% proptosis and > 10- and 11.5-fold enhancement in SOD and catalase values in MCF-7 cells. Cellular uptake was about 100%, 77%, and 0.5% for MCF-7 cells treated with Apt-PLGA-CS-FA, PLGA-CS-FA, and CS nanocarriers, respectively which shows the impact of dual-targeting. The fabricated dual targeted nanodelivery system would be a potential device against breast cancer. © 2025 The Author(s)
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