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Electrophysiological and Molecular Changes Following Neuroprotective Placental Protein Administration on Tinnitus-Induced Rats Publisher



Farhadi M1 ; Gorji A2, 3, 4 ; Mirsalehi M1 ; Poletaev AB5, 6 ; Asadpour A7 ; Mahboudi F8 ; Jafarian M9 ; Farrahizadeh M10 ; Akbarnejad Z1 ; Mahmoudian S1
Authors
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Authors Affiliations
  1. 1. ENT and Head and Neck Research Center, The Five Senses Health Institute, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  2. 2. Epilepsy Research Center, Department of Neurosurgery, Westfalische Wilhelms-Universitat Munster, Munster, Germany
  3. 3. Neuroscience Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
  4. 4. Shefa Neuroscience Research Center, Khatam Alanbia Hospital, Tehran, Iran
  5. 5. Clinical and Research Center of Children Psycho-Neurology, Moscow, Russian Federation
  6. 6. Medical Research Centre “Immunculus�, Moscow, Russian Federation
  7. 7. Intelligent Systems Research Center, Ulster University, Magee Campus, Northern Ireland, Derry~Londonderry, United Kingdom
  8. 8. Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
  9. 9. Brain and Spinal Cord Injury Research Centre, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, Iran
  10. 10. Department of Neuroscience, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran

Source: Laryngoscope Investigative Otolaryngology Published:2023


Abstract

Objective: Despite 6%–20% of the adult population suffering from tinnitus, there is no standard treatment for it. Placenta extract has been used for various therapeutic purposes, including hearing loss. Here, we evaluate the effect of a novel neuroprotective protein composition (NPPC) extract on electrophysiological and molecular changes in the medial geniculate body (MGB) of tinnitus-induced rats. Methods: To evaluate the protein analysis by western blot, the rats were divided into three groups: (1) saline group (intraperitoneal injection of 200 mg/kg saline twice a day for 28 consecutive days, (2) chronic Na-Sal group received sodium salicylate as in the first group, and (3) chronic treatment group (received salicylate 200 mg/kg twice daily for 2 weeks, followed by 0.4 mg NPPC daily from day 14 to day 28). Single-unit recordings were performed on a separate group that was treated as in group 4. Gap-prepulse inhibition of the acoustic startle (GPIAS) and pre-pulse inhibition (PPI) was performed to confirm tinnitus in all groups at the baseline, 14th and 28th days. Results: Western blot analysis showed that the expression of γ-Aminobutyric acid Aα1 subunit (GABA Aα1), N-methyl-d-aspartate receptor subtype 2B (NR2B or NMDAR2B), α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors subunit GluR1 (GluR1), and α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptors subunit GluR2 (GluR2) decreased after Na-Sal injection, while NPPC upregulated their expression. MGB units in rats with tinnitus showed decreased spontaneous firing rate, burst per minute, and a spike in a burst. After NPPC administration, neural activity patterns showed a significant positive effect of NPPC on tinnitus. Conclusion: NPPC can play an effective role in the treatment of tinnitus in salicylate-induced rats, and MGB is one of the brain areas involved in these processes. Level of Evidence: NA. © 2023 The Authors. Laryngoscope Investigative Otolaryngology published by Wiley Periodicals LLC on behalf of The Triological Society.
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