Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Effect of Human Ovarian Tissue Vitrification/Warming on the Expression of Genes Related to Folliculogenesis Publisher Pubmed



Mofarahe ZS1 ; Novin MG1 ; Jafarabadi M2 ; Salehnia M3 ; Noroozian M1 ; Ghorbanmehr N4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Dept. of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  2. 2. Reproductive Health Research Center, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Dept. of Anatomical Sciences, Tarbiat Modares University, Tehran, Iran
  4. 4. Biotechnology Group, Alzahra University, Tehran, Iran

Source: Iranian Biomedical Journal Published:2015


Abstract

Background: Ovarian tissue cryopreservation is an alternative strategy to preserve the fertility of women predicted to undergo premature ovarian failure. This study was designed to evaluate the expression of folliculogenesis-related genes, including factor in the germline alpha (FIGLA), growth differentiation factor-9 (GDF-9), follicle-stimulating hormone receptor (FSHR), and KIT LIGAND after vitrification/warming of human ovarian tissue. Methods: Human ovarian tissue samples were collected from five transsexual women. In the laboratory, the ovarian medullary part was removed by a surgical blade, and the cortical tissue was cut into small pieces. Some pieces were vitrified and warmed and the others were considered as non-vitrified group (control). Follicular normality was assessed with morphological observation by a light microscope, and the expression of FIGLA, KIT LIGAND, GDF- 9,, and FSHR genes was examined using real-time RT-PCR in both the vitrified and non-vitrified groups. Results: Overall, 85% of the follicles preserved their normal morphologic feature after warming. The percentage of normal follicles and the expression of FIGLA, KIT LIGAND, GDF-9, and FSHR genes were similar in both vitrified and non-vitrified groups (P > 0.05). Conclusion: Vitrification/warming of human ovarian tissue had no remarkable effect on the expression of folliculogenesis-related genes. © 2015 Pasteur Institute of Iran. All rights reserved.
Experts (# of related papers)