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Inhibition of Microrna Mir-222 With Lna Inhibitor Can Reduce Cell Proliferation in B Chronic Lymphoblastic Leukemia Publisher



Dehkordi KA1 ; Chaleshtori MH2 ; Sharifi M3 ; Jalili A4 ; Fathi F4 ; Roshani D5 ; Nikkhoo B6 ; Hakhamaneshi MS4 ; Sani MRM7, 8 ; Ganjiarjenaki M9
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Authors Affiliations
  1. 1. Student Research Committee, Kurdistan University of Medical Sciences, Pasdaran Street, Sanandaj, 66177-13446, Iran
  2. 2. Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
  3. 3. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Cellular and Molecular Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran
  5. 5. Social Determinants of Health Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran
  6. 6. Department of Pathology and Clinical Laboratory Sciences, Kurdistan University of Medical Sciences, Sanandaj, Iran
  7. 7. Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
  8. 8. Department of Genetics and Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
  9. 9. Department of Genetics, Faculty of Basic Sciences, Shahrekord University, Shahrekord, Iran

Source: Indian Journal of Hematology and Blood Transfusion Published:2017


Abstract

MicroRNAs (miRNAs) are small regulatory molecules that negatively regulate gene expression by base-pairing with their target mRNAs. miRNAs have contribute significantly to cancer biology and recent studies have demonstrated the oncogenic or tumor-suppressing role in cancer cells. In many tumors up-regulation miRNAs has been reported especially miR-222 has been shown to be up-regulated in B chronic lymphocytic leukemia (B-CLL). In this study we assessed the effected inhibition of miR-222 in cell viability of B-CLL. We performed inhibition of mir-222 in B-CLL cell line (183-E95) using locked nucleic acid (LNA) antagomir. At different time points after LNA-anti-mir-222 transfection, miR-222 quantitation and cell viability were assessed by qRT-real time polymerase chain reaction and MTT assays. The data were analyzed by independent t test and one way ANOVA. Down-regulation of miR-222 in B-CLL cell line (183-E95) with LNA antagomir decreased cell viability in B-CLL. Cell viability gradually decreased over time as the viability of LNA-anti-mir transfected cells was <47 % of untreated cells at 72 h post-transfection. The difference in cell viability between LNA-anti-miR and control groups was statistically significant (p < 0.042). Based on our findings, the inhibition of miR-222 speculate represent a potential novel therapeutic approach for treatment of B-CLL. © 2016, Indian Society of Haematology & Transfusion Medicine.
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