The Effect of Aspirin on Serum Vascular Endothelial Growth Factor and Nitric Oxide Concentration in High-Cholesterol Fed Rabbits



Khazaei M¹ ; Nematbakhsh M² ; Ahmadi H¹ ; Masjedi M³ Show Affiliations
Source: ARYA Atherosclerosis Published:2007

Abstract

INTRODUCTION: Hypercholesterolemia is one of the major risk factors for atherosclerosis which is characterized by endothelial dysfunction. This study was designed to investigate the effect of aspirin on serum vascular endothelial growth factor (VEGF) and nitric oxide (NO) concentrations in hypercholesterolemic animals. METHODS: Sixteen male rabbits were randomly divided into two groups, aspirin-treated and control. Aspirin (10 mg/kg/day) was administered orally using feeding tube. All animals were fed with high-cholesterol diet (1%) during the experiment. After five weeks, blood pressure, serum lipid and lipoprotein profiles, serum VEGF and NO concentrations were measured. RESULTS: Aspirin did not change blood pressure. Aspirin significantly decreased serum LDL (1276±72.1 vs. 1505±68.03 mg/dl) and triglyceride (477.5±8.3 vs. 649.1±15.2 mg/dl) (P<0.05). High-cholesterol diet significantly decreased serum VEGF level in both groups (control: 24.59±0.42 vs. 38.09±2.49 pg/ml; aspirin: 24.72±0.84 vs. 42.29±2.03 pg/ml) (P<0.05) and aspirin did not change serum VEGF level in hypercholesterolemic animals (P>0.05). Serum NO concentration was also significantly decreased after five weeks of high-cholesterol diet (control: 5.87±0.33 vs. 8.67±0.68 µmol/lit; aspirin: 5.66±0.33 vs. 8.58±0.60 µmol/lit) (P<0.05). Aspirin did not change serum NO level (P>0.05). CONCLUSIONS: We conclude that under the conditions of this study, aspirin cannot change serum VEGF and NO concentrations in high-cholesterol fed animals. © 2007, Isfahan University of Medical Sciences(IUMS). All rights reserved.