A Facile One-Step Strategy for Development of a Double Network Fibrous Scaffold for Nerve Tissue Engineering

Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):

Share this content! On (X network) By

A Facile One-Step Strategy for Development of a Double Network Fibrous Scaffold for Nerve Tissue Engineering Publisher Pubmed

Golafshan N¹ ; Gharibi H² ; Kharaziha M¹ ; Fathi M^{1, 3}

Show Affiliations

1. Department of Materials Engineering, Isfahan University of Technology, Isfahan, 84156-83111, Iran
2. Department of Chemistry, Isfahan University of Technology, Isfahan, 84156-83111, Iran
3. Dental Materials Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Biofabrication Published:2017

Abstract

The aim of this study was to develop a novel double network scaffold composed of polycaprolactone fumarate (PCLF) and eggshell membrane (ESM) (ESM:PCLF) by using the vacuum infiltration method. Compared to ESM, the mechanical properties of double network scaffold were significantly improved, depending on the solvents applied for double network scaffold formation; acetic acid and dichloromethane. Noticeably, the toughness and strength of double network scaffold prepared using acetic acid were significantly improved compared to ESM (26.6 and 25 times, respectively) attributed to the existence of hydrophilic functional groups in acetic acid which made ESM flexible to absorb further PCLF solution. To assess the effect of double network formation on the biological behavior of ESM, the attachment, proliferation and spreading of PC12 cells cultured on the ESM:PCLF scaffolds were evaluated. Results revealed that the number of cells attached on double network ESM:PCLF scaffold were nearly similar to ESM and significantly higher than that of on the tissue culture plate (2.6 times) and PCLF film (1.7 times). It is envisioned that the offered ESM:PCLF double network scaffold might have great potential to develop the constructs for nerve regeneration. © 2017 IOP Publishing Ltd.

Related Docs

View other Related Docs

1. Fabrication and Characterization of Polycaprolactone Fumarate/Gelatin-Based Nanocomposite Incorporated With Silicon and Magnesium Co-Doped Fluorapatite Nanoparticles Using Electrospinning Method, Materials Science and Engineering C (2020)

2. The Advances in Nerve Tissue Engineering: From Fabrication of Nerve Conduit to in Vivo Nerve Regeneration Assays, Journal of Tissue Engineering and Regenerative Medicine (2019)

3. Incorporation of Multi-Walled Carbon Nanotubes Into Electrospun Pcl/Gelatin Scaffold: The Influence on the Physical, Chemical and Thermal Properties and Cell Response for Tissue Engineering, Materials Technology (2020)

4. Atorvastatin Lipid Nanocapsules and Gold Nanoparticles Embedded in Injectable Thermogelling Hydrogel Scaffold Containing Adipose Tissue Extracellular Matrix for Myocardial Tissue Regeneration, IET Nanobiotechnology (2019)

5. Poly(Glycerol-Sebacate)/Poly(Caprolactone)/Graphene Nanocomposites for Nerve Tissue Engineering, Journal of Bioactive and Compatible Polymers (2018)

6. An in Vitro and in Vivo Study of Electrospun Polyvinyl Alcohol/Chitosan/Sildenafil Citrate Mat on 3D-Printed Polycaprolactone Membrane As a Double Layer Wound Dressing, International Journal of Biological Macromolecules (2024)

7. Extracellular Matrix Coated Three-Dimensional-Printed Polycaprolactone Scaffolds Containing Curcumin for Cartilage Tissue Engineering Applications, Journal of Bioactive and Compatible Polymers (2024)

8. Fabrication and Characterization of Two-Layered Nanofibrous Membrane for Guided Bone and Tissue Regeneration Application, Materials Science and Engineering C (2017)

Experts (# of related papers)

View all Related Experts

Mohammad Rafienia (10)

Hossein Salehi (9)

Other Related Docs

9. Tunable Cellular Interactions and Physical Properties of Nanofibrous Pcl-Forsterite: Gelatin Scaffold Through Sequential Electrospinning, Composites Science and Technology (2013)

10. A Novel Bilayer Wound Dressing Composed of a Dense Polyurethane/Propolis Membrane and a Biodegradable Polycaprolactone/Gelatin Nanofibrous Scaffold, Scientific Reports (2020)

11. Degradable and Biocompatible Nanofibrous Scaffold Incorporating a Natural Cell Culture Medium for Skin Tissue Engineering, Physica Scripta (2024)

12. Baghdadite/Polycaprolactone Nanocomposite Scaffolds: Preparation, Characterisation, and in Vitro Biological Responses of Human Osteoblast-Like Cells (Saos-2 Cell Line), Materials Technology (2020)

13. Incorporating Fingolimod Through Poly(Lactic-Co-Glycolic Acid) Nanoparticles in Electrospun Polyurethane/Polycaprolactone/Gelatin Scaffold: An in Vitro Study for Nerve Tissue Engineering, Polymers for Advanced Technologies (2022)

14. Incorporation of Nanohydroxyapatite and Vitamin D3 Into Electrospun Pcl/Gelatin Scaffolds: The Influence on the Physical and Chemical Properties and Cell Behavior for Bone Tissue Engineering, Polymers for Advanced Technologies (2018)

15. Cytocompatibility and Antibacterial Properties of Coaxial Electrospun Nanofibers Containing Ciprofloxacin and Indomethacin Drugs, Polymers (2022)

16. Novel Electrospun Polyvinyl Alcohol/Chitosan/Polycaprolactone-Diltiazem Hydrochloride Nanocomposite Membranes for Wound Dressing Applications, Emergent Materials (2024)

17. Preparation and Characterization of Aligned Porous Pcl/Zein Scaffolds As Drug Delivery Systems Via Improved Unidirectional Freeze-Drying Method, Materials Science and Engineering C (2016)

18. In Vitro Hemocompatibility and Cytocompatibility of a Three-Layered Vascular Scaffold Fabricated by Sequential Electrospinning of Pcl, Collagen, and Plla Nanofibers, Journal of Biomaterials Applications (2016)

19. Assessing Physicochemical, Mechanical, and in Vitro Biological Properties of Polycaprolactone/Poly(Glycerol Sebacate)/Hydroxyapatite Composite Scaffold for Nerve Tissue Engineering, Materials Chemistry and Physics (2022)

20. Casein Release and Characterization of Electrospun Nanofibres for Cartilage Tissue Engineering, Bulletin of Materials Science (2022)

21. Metformin-Loaded Pcl/Pva Fibrous Scaffold Preseeded With Human Endometrial Stem Cells for Effective Guided Bone Regeneration Membranes, ACS Biomaterials Science and Engineering (2021)

22. Effects of Cartilage Acellular Solubilised Ecm on Physicomechanical and Biological Properties of Polycaprolactone/Fibrin Hybrid Scaffold Fabricated by 3D-Printing and Salt-Leaching Methods, Materials Technology (2022)

23. Characterization of Poly(Ε-Caprolactone)/Extracellular Matrix Nanofibers Composite Scaffold for Tissue Engineering, Journal of Isfahan Medical School (2019)

24. Electrospun Polyurethane-Gelatin Composite: A New Tissue-Engineered Scaffold for Application in Skin Regeneration and Repair of Complex Wounds, ACS Biomaterials Science and Engineering (2020)

25. Does the Tissue Engineering Architecture of Poly(3-Hydroxybutyrate) Scaffold Affects Cell-Material Interactions?, Journal of Biomedical Materials Research - Part A (2012)

26. Pharmaceutical Evaluation of Atorvastatin-Loaded Nanostructured Lipid Carriers Incorporated Into the Gelatin/Hyaluronic Acid/Polycaprolactone Scaffold for the Skin Tissue Engineering, Journal of Biomaterials Applications (2021)

27. Natural and Synthetic Polymeric Scaffolds Used in Peripheral Nerve Tissue Engineering: Advantages and Disadvantages, Polymers for Advanced Technologies (2021)

28. Electrospun Polycaprolactone/Gelatin/Bioactive Glass Nanoscaffold for Bone Tissue Engineering, International Journal of Polymeric Materials and Polymeric Biomaterials (2019)

29. The Thickness of Electrospun Poly (Ε-Caprolactone Nanofibrous Scaffolds Influences Cell Proliferation, International Journal of Artificial Organs (2009)

30. A Ternary Nanocomposite Fibrous Scaffold Composed of Poly(Ε-Caprolactone)/Gelatin/Gehlenite (Ca2al2sio7): Physical, Chemical, and Biological Properties in Vitro, Polymers for Advanced Technologies (2021)

31. Coaxial Electrospun Pgs/Pcl and Pgs/Pgs-Pcl Nanofibrous Membrane Containing Platelet-Rich Plasma for Skin Tissue Engineering, Journal of Biomaterials Science, Polymer Edition (2024)

32. Development of an Electrospun Poly(Ε-Caprolactone)/Collagen-Based Human Amniotic Membrane Powder Scaffold for Culturing Retinal Pigment Epithelial Cells, Scientific Reports (2022)

33. Nanofibrous Scaffolds With Biomimetic Composition for Skin Regeneration, Applied Biochemistry and Biotechnology (2019)

34. Adipose-Derived Stem Cells Growth and Proliferation Enhancement Using Poly (Lactic-Co-Glycolic Acid) (Plga)/Fibrin Nanofiber Mats, Journal of Applied Biotechnology Reports (2021)

35. Polyglycerol Sebacate/Polycaprolactone/Reduced Graphene Oxide Composite Scaffold for Myocardial Tissue Engineering, Heliyon (2024)

36. Pcl/Agarose 3D-Printed Scaffold for Tissue Engineering Applications: Fabrication, Characterization, and Cellular Activities, Research in Pharmaceutical Sciences (2023)

37. Electrospun Aligned Plga and Plga/Gelatin Nanofibers Embedded With Silica Nanoparticles for Tissue Engineering, International Journal of Biological Macromolecules (2015)

38. Cartilage Particles Can Promote Chondrogenesis of Adipose-Derived Stromal Cells on Poly(Ε-Caprolactone)/Fibrin Hybrid Constructs Prepared Via Sandwich Model, Journal of Biomimetics, Biomaterials and Biomedical Engineering (2020)

39. Novel Bilayer Electrospun Poly(Caprolactone)/ Silk Fibroin/ Strontium Carbonate Fibrous Nanocomposite Membrane for Guided Bone Regeneration, Journal of Applied Polymer Science (2021)

40. Preparation and Characterization of Carboxymethyl Cellulose/Polyethylene Glycol Films Containing Bromelain/Curcumin: In Vitro Evaluation of Wound Healing Activity, Polymer Engineering and Science (2024)

41. The Effect of Aligned and Random Pcl-Human Amniotic Membrane Powder Scaffolds on Retinal Tissue Engineering, Advances in Materials Science and Engineering (2023)

42. In Vitro Comparison Study of Plasma Treated Bilayer Pgs/Pcl and Pgs/Pla Scaffolds for Vascular Tissue Engineering, Fibers and Polymers (2022)

43. A Bifunctional Electrospun Nanocomposite Wound Dressing Containing Surfactin and Curcumin: In Vitro and in Vivo Studies, Materials Science and Engineering C (2021)

44. Bioceramic Calcium Phosphate-Polymer Scaffolds: A Promising Strategy for Osteochondral Repair and Regenerative Medicine, Materials Chemistry and Physics (2023)

45. Poly (Ethylene-Co-Vinyl Alcohol)-Based Polymeric Thermo-Responsive Nanocarriers for Controlled Delivery of Epirubicin to Hepatocellular Carcinoma, Research in Pharmaceutical Sciences (2017)

46. In Vitro Study of the Recruitment and Expansion of Mesenchymal Stem Cells at the Interface of a Cu-Doped Pcl-Bioglass Scaffold, Biomimetics (2022)

47. Sustained Release Investigation of Curcumin and Ciprofloxacin on Coaxial Electrospun Nanocomposite Scaffold of Poly (3-Hydroxybutyrate)-Poly Glycerol Sebacate: An Approach for Skin Regeneration, Emergent Materials (2024)

48. Anti-Tumor Effects of Ellagic Acid and Mesenchymal Stem Cell-Conditioned Medium on Breast Cancer Cells on the 3D Printed Pcl/Agarose Scaffolds, Journal of Drug Delivery Science and Technology (2024)

49. Fabrication and Characterization of Pcl/Gel Nanofibrous Scaffolds Incorporated With Graphene Oxide Applicable in Cardiac Tissue Engineering, Polymer-Plastics Technology and Materials (2021)

50. Synthetic-Based Blended Electrospun Scaffolds in Tissue Engineering Applications, Journal of Materials Science (2022)

Style	Citing Format
MLA	Golafshan N, et al.. "A Facile One-Step Strategy for Development of a Double Network Fibrous Scaffold for Nerve Tissue Engineering." Biofabrication, vol. 9, no. 2, 2017, pp. -.
APA	Golafshan N, Gharibi H, Kharaziha M, Fathi M (2017). A Facile One-Step Strategy for Development of a Double Network Fibrous Scaffold for Nerve Tissue Engineering. Biofabrication, 9(2), -.
Chicago	Golafshan N, Gharibi H, Kharaziha M, Fathi M. "A Facile One-Step Strategy for Development of a Double Network Fibrous Scaffold for Nerve Tissue Engineering." Biofabrication 9, no. 2 (2017): -.
Harvard	Golafshan N et al. (2017) 'A Facile One-Step Strategy for Development of a Double Network Fibrous Scaffold for Nerve Tissue Engineering', Biofabrication, 9(2), pp. -.
Vancouver	Golafshan N, Gharibi H, Kharaziha M, Fathi M. A Facile One-Step Strategy for Development of a Double Network Fibrous Scaffold for Nerve Tissue Engineering. Biofabrication. 2017;9(2):-.
BibTex	@article{ author = {Golafshan N and Gharibi H and Kharaziha M and Fathi M}, title = {A Facile One-Step Strategy for Development of a Double Network Fibrous Scaffold for Nerve Tissue Engineering}, journal = {Biofabrication}, volume = {9}, number = {2}, pages = {-}, year = {2017} }
RIS	TY - JOUR AU - Golafshan N AU - Gharibi H AU - Kharaziha M AU - Fathi M TI - A Facile One-Step Strategy for Development of a Double Network Fibrous Scaffold for Nerve Tissue Engineering JO - Biofabrication VL - 9 IS - 2 SP - EP - PY - 2017 ER -