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In Vitro Study of the Recruitment and Expansion of Mesenchymal Stem Cells at the Interface of a Cu-Doped Pcl-Bioglass Scaffold Publisher

Summary: Electrospun PCL/gelatin membranes enriched with bioglass or Cu-doped bioglass improve aBMSC proliferation, bioactivity, and biomechanical properties, supporting periodontal tissue regeneration. #TissueEngineering #Periodontology #Biomaterials

Malekahmadi B1, 2 ; Esfahanian V3 ; Ejeian F2 ; Dastgurdi ME4 ; Agheb M5 ; Kaveian F6 ; Rafienia M6 ; Nasresfahani MH2
Authors

Source: Biomimetics Published:2022


Abstract

Developing new barrier membranes with improved biomechanical characteristics has acquired much interest owing to their crucial role in the field of periodontal tissue regeneration. In this regard, we enriched the electrospun polycaprolactone (PCL)/gelatin (Gel) membranes by adding bioglass (BG) or Cu-doped bioglass (CuBG) and examined their cellular adhesion and proliferation potential in the presence of alveolar bone marrow-derived mesenchymal stem cells (aBMSCs). The membranes were fabricated and characterized using mechanical strength, SEM, FTIR, EDX, and ICP assay. Besides, aBMSCs were isolated, characterized, and seeded with a density of 35,000 cells in each experimental group. Next, the cellular morphology, cell adhesion capacity, proliferation rate, and membrane antibacterial activity were assessed. The results displayed a significant improvement in the wettability, pore size, and Young’s modulus of the PCL membrane following the incorporation of gelatin and CuBG particles. Moreover, all scaffolds exhibited reasonable biocompatibility and bioactivity in physiological conditions. Although the PCL/Gel/CuBG membrane revealed the lowest primary cell attachment, cells were grown properly and reached the confluent state after seven days. In conclusion, we found a reasonable level of attachment and proliferation of aBMSCs on all modified membranes. Meanwhile, a trace amount of Cu provided superiority for PCL/Gel/CuBG in periodontal tissue regeneration. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
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