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Amorphous Magnesium Phosphate-Graphene Oxide Nano Particles Laden 3D-Printed Chitosan Scaffolds With Enhanced Osteogenic Potential and Antibacterial Properties Publisher Pubmed



Pahlevanzadeh F1 ; Emadi R1 ; Kharaziha M1 ; Poursamar SA2 ; Nejatidanesh F3 ; Emadi H1 ; Aslani R2 ; Moroni L4 ; Setayeshmehr M5
Authors
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Authors Affiliations
  1. 1. Department of Materials Engineering, Isfahan University of Technology, Isfahan, 84156-83111, Iran
  2. 2. Department of Biomaterials, Nanotechnology and Tissue Engineering, School of Advanced Technologies in Medicine, Isfahan University of Medical Sciences, Isfahan, 81746-73461, Iran
  3. 3. Dental Materials Research Center, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Complex Tissue Regeneration Department, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Maastricht, Netherlands
  5. 5. Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, 81746-73461, Iran

Source: Biomaterials Advances Published:2024


Abstract

The utilization of 3D printing technology for the fabrication of graft substitutes in bone repair holds immense promise. However, meeting the requirements for printability, bioactivity, mechanical strength, and biological properties of 3D printed structures concurrently poses a significant challenge. In this study, we introduce a novel approach by incorporating amorphous magnesium phosphate-graphene oxide (AMP-GO) into a thermo-crosslinkable chitosan/β glycerol phosphate (CS/GP) ink. We fabricated thermo-crosslinkable CS inks containing varying concentrations (10 %, 20 %, or 30 % weight) of AMP-GO. The 3D printed scaffolds incorporating 20 % AMP-GO exhibited significantly improved mechanical properties, with compressive strengths of 4.5 ± 0.06 MPa compared to 0.5 ± 0.03 MPa for CS printed scaffolds. Moreover, the CS/AMP-GO inks demonstrated enhanced antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) bacteria, attributed to the release of magnesium cations and the performance of GO. Additionally, CS/20AMP-GO ink facilitated increased adhesion, viability, proliferation, and osteogenic differentiation of mesenchymal stem cells (MSCs), as evidenced by the upregulation of ALP, COL1, and Runx2 expression, which were elevated 9.8, 6.5, and >22 times, respectively, compared to pure CS scaffolds. Considering its exceptional in vivo osteogenic potential, we anticipate that the CS/20AMP-GO ink holds great potential for 3D printing of bone grafts. © 2024 Elsevier B.V.
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