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Aptamer Modified Nanoprobe for Multimodal Fluorescence/Magnetic Resonance Imaging of Human Ovarian Cancer Cells Publisher



Asgari M1 ; Khanahmad H2 ; Motaghi H3 ; Farzadniya A4 ; Mehrgardi MA3 ; Shokrani P5
Authors
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Authors Affiliations
  1. 1. Department of Nursing, School of Nursing, Larestan University of Medical Sciences, Larestan, Iran
  2. 2. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Chemistry, University of Isfahan, Isfahan, Iran
  4. 4. Department of Radiology, Askarieh Hospital, Isfahan, Iran
  5. 5. Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Applied Physics A: Materials Science and Processing Published:2021


Abstract

A multifunctional-aptamer (APT) nanoprobe, TOV6 APT-superparamagnetic iron oxide nanoparticle-carbon dots (APT-SPION-CDs), for fluorescence and magnetic resonance targeted imaging (FI/MRI) of human ovarian cancer cells (OVCAR-3) is introduced. The SPION-CDs were synthesized and conjugated with TOV6 APT recognizing the stress-induced phosphoprotein 1 (STIP1) overexpressed on OVCAR-3 cells. The capability of the nanoprobe as a contrast agent for MRI and simultaneously as a fluorescent probe for fluorescence microscopy was studied. TOV6 APTs were adsorbed on SPION-CDs and were confirmed with Fourier transform infrared spectrometer. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay showed higher cytotoxic effects for APT-SPION-CDs compared to SPION-CDs on STIP1-negative HeLa cells. But on OVCAR-3, as STIP1-positive cells, the cytotoxic effect was negligible. The fluorescence microscopic images confirmed that APT-SPION-CDs specifically targeted ovarian cancer cells due to the tumor-targeting effect of TOV6 APT. High spin–spin relaxivity value (r2 = 308.5 mM−1 s−1) and the signal enhancement in T2-weighted MRIs confirmed the capability of the nanoprobe as a T2-based contrast agent. In vitro cellular uptake and signal enhancement of this multimodal FI/MRI nanoprobe demonstrated the potential application of APT-SPION-CDs as a contrast agent for MRI and as a fluorescent probe for fluorescence microscopic imaging. © 2021, Springer-Verlag GmbH Germany, part of Springer Nature.
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