Co-Treatment With Sulforaphane and Nano-Metformin Molecules Accelerates Apoptosis in Her2+ Breast Cancer Cells by Inhibiting Key Molecules

Co-Treatment With Sulforaphane and Nano-Metformin Molecules Accelerates Apoptosis in Her2+ Breast Cancer Cells by Inhibiting Key Molecules Publisher Pubmed

Keshandehghan A¹ ; Nikkhah S² ; Tahermansouri H² ; Heidarikeshel S³ ; Gardaneh M¹

Show Affiliations

1. Department of Stem Cells and Regenerative Medicine, Division of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
2. Department of Chemistry, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
3. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Nutrition and Cancer Published:2020

Abstract

Breast cancer cell lines MCF-10, MCF-7 and BT-474 expressing various levels of HER2 were examined for their response to treatment with sulforaphane (SLFN), metformin (MTFN), Nano-MTFN or combinations. Direct correlation was found between SLFN effect on cell death and HER2 levels. Bioinformatic studies suggested the possibility of additive co-effects on cell fate by SLFN-MTFN co-treatment. This co-treatment specially with SLFN + Nano-MTFN significantly affected the survival of the cells and killed more BT-474 cells than the other two. Cell sensitivity to SLFN-MTFN combination correlated with HER2 expression levels. RT-PCR showed that parallel with cell death, expression of BCL-2, SRC, WNT1, β-catenin and CD44 are diminished, whereas BAX levels are elevated significantly. Cell co-staining indicated that apoptosis percent correlates with cell death following different treatments. We also found that cell death induced by SLFN-MTFN co-treatment is in direct correlation with HER2 levels and increased cell death correlates directly with BAX levels but inversely with levels of cancer stem cell (CSC) signaling genes and CD44. In conclusion, our data indicate that SLFN and MTFN can reduce cancer cell viability via both collaborative and differential effects and suggest that MTFN increases SLFN effectiveness by targeting common molecules/pathways downstream of HER2 and key for CSC signaling. © 2019, © 2019 Taylor & Francis Group, LLC.

Related Docs

View other Related Docs

1. Metformin: Prevention of Genomic Instability and Cancer: A Review, Mutation Research - Genetic Toxicology and Environmental Mutagenesis (2018)

2. Metformin and Long Non-Coding Rnas in Breast Cancer, Journal of Translational Medicine (2023)

3. Synergistic Anticancer Effects of Doxorubicin and Metformin Combination Therapy: A Systematic Review, Translational Oncology (2024)

4. Influence of Functionalised Multiwalled Carbon Nanotubes With Imidazole Derivative and Thiosemicarbazide on Mkn45 and Sw742 Cancer Cells, Materials Technology (2015)

5. Gdnf Induces Ret–Src–Her2-Dependent Growth in Trastuzumab-Sensitive But Src-Independent Growth in Resistant Breast Tumor Cells, Breast Cancer Research and Treatment (2017)

6. Synthesis, Characterization, and Toxicity of Multi-Walled Carbon Nanotubes Functionalized With 4-Hydroxyquinazoline, Carbon Letters (2016)

7. Impact of Metformin on the Therapeutic Effect of Radiotherapy, Radiation Medicine and Protection (2021)

8. The Effect of Metformin on Survival Outcomes of Non-Metastatic Breast Cancer Patients With Type 2 Diabetes, Asian Pacific Journal of Cancer Prevention (2021)

Experts (# of related papers)

View all Related Experts

Mahsa Mohamad Amoli (2)

Mohammad Ali Mohagheghi (1)

Style	Citing Format
MLA	Keshandehghan A, et al.. "Co-Treatment With Sulforaphane and Nano-Metformin Molecules Accelerates Apoptosis in Her2+ Breast Cancer Cells by Inhibiting Key Molecules." Nutrition and Cancer, vol. 72, no. 5, 2020, pp. 835-848.
APA	Keshandehghan A, Nikkhah S, Tahermansouri H, Heidarikeshel S, Gardaneh M (2020). Co-Treatment With Sulforaphane and Nano-Metformin Molecules Accelerates Apoptosis in Her2+ Breast Cancer Cells by Inhibiting Key Molecules. Nutrition and Cancer, 72(5), 835-848.
Chicago	Keshandehghan A, Nikkhah S, Tahermansouri H, Heidarikeshel S, Gardaneh M. "Co-Treatment With Sulforaphane and Nano-Metformin Molecules Accelerates Apoptosis in Her2+ Breast Cancer Cells by Inhibiting Key Molecules." Nutrition and Cancer 72, no. 5 (2020): 835-848.
Harvard	Keshandehghan A et al. (2020) 'Co-Treatment With Sulforaphane and Nano-Metformin Molecules Accelerates Apoptosis in Her2+ Breast Cancer Cells by Inhibiting Key Molecules', Nutrition and Cancer, 72(5), pp. 835-848.
Vancouver	Keshandehghan A, Nikkhah S, Tahermansouri H, Heidarikeshel S, Gardaneh M. Co-Treatment With Sulforaphane and Nano-Metformin Molecules Accelerates Apoptosis in Her2+ Breast Cancer Cells by Inhibiting Key Molecules. Nutrition and Cancer. 2020;72(5):835-848.
BibTex	@article{ author = {Keshandehghan A and Nikkhah S and Tahermansouri H and Heidarikeshel S and Gardaneh M}, title = {Co-Treatment With Sulforaphane and Nano-Metformin Molecules Accelerates Apoptosis in Her2+ Breast Cancer Cells by Inhibiting Key Molecules}, journal = {Nutrition and Cancer}, volume = {72}, number = {5}, pages = {835-848}, year = {2020} }
RIS	TY - JOUR AU - Keshandehghan A AU - Nikkhah S AU - Tahermansouri H AU - Heidarikeshel S AU - Gardaneh M TI - Co-Treatment With Sulforaphane and Nano-Metformin Molecules Accelerates Apoptosis in Her2+ Breast Cancer Cells by Inhibiting Key Molecules JO - Nutrition and Cancer VL - 72 IS - 5 SP - 835 EP - 848 PY - 2020 ER -

Science Communicator Platform

Authors

Authors Affiliations

Abstract