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Human Umbilical Cord Mesenchymal Stem Cells-Derived Exosomes Can Alleviate the Proctitis Model Through Tlr4/Nf-Κb Pathway Publisher



Soltani ZE1, 2 ; Elahi M1, 3 ; Golroudbari HT1, 4 ; Badripour A4 ; Nazari H5 ; Heiranitabasi A1 ; Asbagh RA1, 4 ; Ohadi MAD1, 4 ; Shabani M1 ; Mahboudi M1 ; Sarzaeim M4, 5 ; Mahdavi SA3 ; Behboudi B2 ; Keramati MR2 Show All Authors
Authors
  1. Soltani ZE1, 2
  2. Elahi M1, 3
  3. Golroudbari HT1, 4
  4. Badripour A4
  5. Nazari H5
  6. Heiranitabasi A1
  7. Asbagh RA1, 4
  8. Ohadi MAD1, 4
  9. Shabani M1
  10. Mahboudi M1
  11. Sarzaeim M4, 5
  12. Mahdavi SA3
  13. Behboudi B2
  14. Keramati MR2
  15. Kazemeini A2
  16. Tafti SMA2
Show Affiliations
Authors Affiliations
  1. 1. Research Center for Advanced Technologies in Cardiovascular Medicine, Cardiovascular Research Institute, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Colorectal Research Center, Imam Khomeini Hospital Complex, Tehran University of Medical Science, Tehran, Iran
  3. 3. School of Medicine, Shahid Beheshti University of Medical Science, Tehran, Iran
  4. 4. School of Medicine, Tehran University of Medical Science, Tehran, Iran
  5. 5. School of Biomedical Engineering, University of Technology Sydney, Sydney, 2007, NSW, Australia

Source: Journal of Cellular and Molecular Anesthesia Published:2023


Abstract

Background: Proctitis is a significant concern of inflammatory bowel diseases, especially ulcerative colitis. Exosomes are a new method for treating many diseases by their immunosuppressive and tissue-repairing potential. Here, we tried Mesenchymal stem cells (MSCs)-derived Exosomes for treating the proctitis model of rats. Materials and Methods: Rats were assigned into four groups: sham, control group, rectal, and intraperitoneal exosome injection. The proctitis model was induced by rectal administration of 4% acetic acid. The exosome was derived from human MSCs isolated from human umbilical cords. After seven days, rectum samples were assessed for histopathological, IHC, and PCR analysis. Results: The histopathologic scores, collagen deposition, and the expression of NF-κB, TLR4, TNFα, IL-6, and TGFβ were decreased in intraperitoneal exosome compared to controls. The result was not promising for the rectal administration of exosomes. Conclusion: Exosomes can suppress the inflammatory response in the proctitis model and improve the rectum's healing process. Exosomes can inhabit the NF-κB/TLR4 pathway and downstream pro-inflammatory cytokines. This study implicates the therapeutic benefits of exomes in treating proctitis. © 2023 Shahid Beheshti University of Medical Sciences, Anesthesiology Research Center. All rights reserved.