Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Dna Methyltransferase Activity Detection Based on Graphene Quantum Dots Using Fluorescence and Fluorescence Anisotropy Publisher



Kermani HA1 ; Hosseini M1 ; Dadmehr M2 ; Hosseinkhani S3 ; Ganjali MR4, 5
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Life Science Engineering, Faculty of New Sciences & Technologies, University of Tehran, Tehran, Iran
  2. 2. Department of Biotechnology, Payame Noor University, Tehran, Iran
  3. 3. Department of Biochemistry, Tarbiat Modares University, Tehran, Iran
  4. 4. Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, Iran
  5. 5. Biosensor Research Center, Endocrinology & Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: Sensors and Actuators# B: Chemical Published:2017


Abstract

DNA methyltransferase enzyme has a crucial role in many biological processes. Abnormal expression level of this enzyme has been regarded as a predictive cancer biomarker. Herein a novel method based on DNA modified GQDs was developed for fluorescence based assay and anisotropic investigation of M.SssI activity. An amine modified ds-DNA was designed including a recognition site for both methyltransferase M.SssI and endonuclease HpaII. By conjugation of ds-DNA to GQDs, fluorescence was decreased to 45%. When M.SssI was introduced, ds-DNA was methylated at palindromic sequence 5'…CCGG…3' and become resistant to cleavage by HpaII and no change was observed in fluorescence intensity. Contrarily without sufficient M.SssI enzyme, HpaII cleaved DNA strands and induced enhancement of fluorescence. The proposed method exhibited a low detection limit of 0.7 U/ml. Additional fluorescence anisotropy method was performed to confirm the DNA modification and release of DNA strands from the surface of GQDs in the presence of M.SssI and HpaII respectively. The application of the assay in the serum sample was successfully investigated. This simple method can be a useful tool to apply in diagnosis and biomedical research. © 2016 Elsevier B.V.
Related Docs
View other Related Docs
1. A Fluorometric Study on the Effect of Dna Methylation on Dna Interaction With Graphene Quantum Dots, Methods and Applications in Fluorescence (2019)
2. A Colorimetric Assay of Dna Methyltransferase Activity Based on Peroxidase Mimicking of Dna Template Ag/Pt Bimetallic Nanoclusters, Analytical and Bioanalytical Chemistry (2018)
3. Application of Intercalating Molecules in Detection of Methylated Dna in the Presence of Silver Ions, Methods and Applications in Fluorescence (2019)
4. Cerium(Iii) Ion Sensing Based on Graphene Quantum Dots Fluorescent Turn-Off, Journal of Fluorescence (2017)
5. Synthesis, Properties and Biomedical Applications of Carbon-Based Quantum Dots: An Updated Review, Biomedicine and Pharmacotherapy (2017)
6. Methylation in Colorectal Cancer, Epigenetics Territory and Cancer (2015)
7. Fluorimetric Detection of Methylated Dna of Sept9 Promoter by Silver Nanoclusters at Intrastrand 6C-Loop, Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy (2021)
8. Epi-Drugs and Epi-Mirs: Moving Beyond Current Cancer Therapies, Current Cancer Drug Targets (2016)
Experts (# of related papers)
View all Related Experts
Hamid Reza Mirzaei (2)
Babak Negahdari (1)
Other Related Docs
9. Potential Use of Dok7 Gene Methylation Level in the Peripheral Blood Cells As a Biomarker in Early Diagnosis of Sporadic Breast Cancer, Genetics in the Third Millennium (2016)
10. Fluorescent Apta-Nanobiosensors for Fast and Sensitive Detection of Digoxin in Biological Fluids Using Rgqds: Comparison of Two Approaches for Immobilization of Aptamer, Sensors and Actuators# B: Chemical (2020)
11. Label-Free Fluorescent Detection of Microrna-155 Based on Synthesis of Hairpin Dna-Templated Copper Nanoclusters by Etching (Top-Down Approach), Sensors and Actuators# B: Chemical (2017)
12. On-Chip Analysis of Carbon Dots Effect on Yeast Replicative Lifespan, Analytica Chimica Acta (2018)
13. Phytochemicals Alleviate Tumorigenesis by Regulation of M1/M2 Polarization: A Systematic Review of the Current Evidence, Phytotherapy Research (2025)
14. A New Fluorescence Turn-On Nanobiosensor for the Detection of Micro-Rna-21 Based on a Dna-Gold Nanocluster, Methods and Applications in Fluorescence (2017)
15. Sirna and Epigenetic Aberrations in Ovarian Cancer, Journal of Cancer Research and Therapeutics (2016)
16. Fluorometric Determination of Microrna Via Fret Between Silver Nanoclusters and Cdte Quantum Dots, Microchimica Acta (2017)
17. Future Challenges and Prospects for the Epigenetics of Autoimmunity, The Epigenetics of Autoimmunity (2018)
18. Colorimetric Aptasensor for Campylobacter Jejuni Cells by Exploiting the Peroxidase Like Activity of Au@Pd Nanoparticles, Microchimica Acta (2018)
19. Detection of P53 Gene Mutation (Single-Base Mismatch) Using a Fluorescent Silver Nanoclusters, Journal of Fluorescence (2017)
20. Synthesis of Fluorescent Cysteine-Gold Nano-Clusters (Cys-Au-Ncs) and Their Application As Nano-Biosensors for the Determination of Cysteine, Current Nanoscience (2017)
21. Novel Fluorometric Assay for Detection of Cysteine As a Reducing Agent and Template in Formation of Copper Nanoclusters, Journal of Fluorescence (2017)
22. Biomarker Sensing Using Luminescent Metal Nanoclusters, Luminescent Metal Nanoclusters: Synthesis# Characterization# and Applications (2022)
23. Whole Cell Fret Immunosensor Based on Graphene Oxide and Graphene Dot for Campylobacter Jejuni Detection, Food Chemistry (2020)
24. Detection of Large Deletion in Human Brca1 Gene in Human Breast Carcinoma Mcf-7 Cells by Using Dna-Silver Nanoclusters, Methods and Applications in Fluorescence (2018)
25. New Insight Into G-Quadruplexes; Diagnosis Application in Cancer, Analytical Biochemistry (2021)
26. Epigenetic Control of Atherosclerosis Via Dna Methylation: A New Therapeutic Target?, Life Sciences (2020)
27. Fluorescence Based Turn-On Strategy for Determination of Microrna-155 Using Dna-Templated Copper Nanoclusters, Microchimica Acta (2017)