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Application of Intercalating Molecules in Detection of Methylated Dna in the Presence of Silver Ions Publisher Pubmed



Soltaninejad H1 ; Sadeghan AA2 ; Hosseinkhani S2, 6 ; Asadollahi MA1 ; Hosseini M3 ; Ganjali MR4, 5
Authors
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Authors Affiliations
  1. 1. Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, Iran
  2. 2. Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
  3. 3. Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran
  4. 4. Center of Excellence in Electrochemistry, University of Tehran, Tehran, Iran
  5. 5. Biosensor Research Center, Endocrinology and Metabolism Molecular - Cellular Sciences Institute, Tehran, Iran
  6. 6. Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Methods and Applications in Fluorescence Published:2019


Abstract

The extent of DNA structural perturbation by silver ions is different in methylated and non-methylated DNA. Here, we explored the interaction of eight convenient DNA interacting molecules with methylated and non-methylated short GC rich oligonucleotides in the presence and absence of silver ions. Acridine orange, DAPI, Doxorubicin, Ethidium bromide, Hoechst 33342, Methylene blue, PicoGreen, and Propidium iodide are tested for their ability to distinguish methylated and non-methylated DNA. Among them, Ethidium bromide, Methylene blue, and PicoGreen were able to discriminate between methylated and non-methylated DNA, while DAPI and Hoechst 33342 were only able to discriminate with the aid of silver ions. A detection method is proposed using Ethidium bromide in which the silver-treated sample of DNA exposed different fluorescence intensity from the untreated one on the base of its methylation state. This phenomenon was sequence-dependent and could provide a sensing platform with a detection limit of about 4fi0 nM. © 2019 IOP Publishing Ltd.
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