Plasmonic Photothermal Therapy of Colon Cancer Cells Utilising Gold Nanoshells: An in Vitro Study Publisher



Koohi SR¹ ; Derakhshan MA¹ ; Faridani F² ; Nejad SM³ ; Amanpour S⁴ ; Tajerian R¹ ; Yarmahmoodi M⁵ ; Faridimajidi R¹
Source: IET Nanobiotechnology Published:2018

Abstract

In this study, gold nanoshell (GNS) were synthesised utilising the Halas method. The obtained nanoparticles (NPs) were characterised by Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), UV–Vis spectroscopy and dynamic light scattering. FTIR spectra demonstrated the successful functionalisation of silica NP with 3-aminopropyl trimethoxysilane. SEM and TEM images showed the morphology and diameter of the synthesised silica NPs (137 ± 26 nm) and GNS. UV–Vis spectrum illustrated the maximum absorbance of the resultant GNS and their average hydrodynamic diameter was 159 nm. For in vitro study, HCT-116 cells were exposed to gold nanoshells and intense pulsed light in different experiment groups. The results showed that exposing the cells to nanoshells and 30 s irradiation would efficiently decrease the viability percentage of the cells to about 30% compared with the control. A continued exposure of 4 min decreased the viability of the cancer cells to 20%. The results demonstrated that photothermal therapy would be promising in treatment of colon cancer cells utilising gold nanoshells. © The Institution of Engineering and Technology 2017.