Enhanced Peroxidase-Like Activity of Platinum Nanoparticles Decorated on Nickel- and Nitrogen-Doped Graphene Nanotubes: Colorimetric Detection of Glucose Publisher Pubmed



Fakhri N¹ ; Salehnia F² ; Mohammad Beigi S² ; Aghabalazadeh S³ ; Hosseini M⁴ ; Ganjali MR^{2, 5} Show Affiliations
Source: Microchimica Acta Published:2019

Abstract

A nanostructured catalyst is introduced that demonstrates peroxidase mimicking activity. It consists of nickel- and nitrogen-doped graphene nanotubes loaded with platinum nanoparticles. Pt-decorated Ni-doped nitrogen-rich graphitic nanotube (Pt/Ni@NGT) was synthesized using a two-step procedure in which the precursors were first refluxed to form a supramolecular assembly followed by a pyrolysis and leaching step to form nanotubes. Afterwards, Pt was decorated on the outer surface of nanotube by an ultrasound assisted method. Pt/Ni@NGT was characterized by XPS, TEM, SEM, and HAADF–STEM. The as-prepared Pt/Ni@NGT nanostructure was used for the detection of glucose via catalyzing the oxidation of a substrate, 3,3′,5,5′-tetramethylbenzidine (TMB), to form a blue product (ox-TMB), thereby enabling colorimetric assay for enzymatically generated H2O2. The nanostructure exhibited excellent biocompatibility and led to highly efficient immobilization and retention of GOx. The method has a linear response in the 43 pM to 220 μM glucose concentration range, a detection limit as low as 1 pM and a limit of quantification of 3.4pM, along with good reproducibility(< 3%). A paper based visual microfluidic assay was also worked out that has an analytical range that extends from 0.1–50 mM. It is simple and rapid enough to be useful as a glucose home test. The method was successfully applied to the determination of glucose in tear and saliva samples. [Figure not available: see fulltext.]. © 2019, Springer-Verlag GmbH Austria, part of Springer Nature.