Serotonin Level As a Potent Diabetes Biomarker Based on Electrochemical Sensing: A New Approach in a Zebra Fish Model

Serotonin Level As a Potent Diabetes Biomarker Based on Electrochemical Sensing: A New Approach in a Zebra Fish Model Publisher Pubmed

Khoshnevisan K^{1, 2, 3} ; Baharifar H⁴ ; Torabi F⁶ ; Sadeghi Afjeh M² ; Maleki H⁷ ; Honarvarfard E⁸ ; Mohammadi H³ ; Sajjadijazi SM^{2, 9} ; Mahmoudikohan S⁶ ; Faridbod F⁶ ; Larijani B² ; Saadat F¹⁰ ; Faridi Majidi R⁷ ; Khorramizadeh MR^{1, 2, 3}

Show Affiliations

1. Biosensor Research Center, Endocrinology & Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, 1411713137, Iran
2. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, 1411713137, Iran
3. Zebrafish Core Facility, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, 1411713137, Iran
4. Department of Medical Nanotechnology, Applied Biophotonics Research Center, Science and Research Branch, Islamic Azad University, Tehran, 1477893855, Iran
5. School of Chemistry, College of Science, University of Tehran, Tehran, 1417466191, Iran
6. Center of Excellence in Electrochemistry, School of Chemistry, College of Science, University of Tehran, Tehran, 1417466191, Iran
7. Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, 1417755469, Iran
8. Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, 13699-5810, NY, United States
9. Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, 1411713137, Iran
10. Department of Immunology, School of Medicine, Guilan University of Medical Sciences, Rasht, 41887-94755, Iran

Source: Analytical and Bioanalytical Chemistry Published:2021

Abstract

Serotonin (5-HT) levels have been associated with several exclusively metabolic disorders. Herein, a new approach for 5-HT level as a novel biomarker of diabetes mellitus is considered using a simple nanocomposite and HPLC method. Reduced graphene oxide (rGO) comprising gold nanoparticles (AuNPs) was decorated with 18-crown-6 (18.Cr.6) to fabricate a simple nanocomposite (rGO-AuNPs-18.Cr.6). The nanocomposite was positioned on a glassy carbon electrode (GCE) to form an electrochemical sensor for the biomarker 5-HT in the presence of L-tryptophan (L-Trp), dopamine (DA), ascorbic acid (AA), urea, and glucose. The nanocomposite exhibited efficient catalytic activity for 5-HT detection by square-wave voltammetry (SWV). The proposed sensor displayed high selectivity, excellent reproducibility, notable anti-interference ability, and long-term stability even after 2 months. SWV defined a linear range of 5-HT concentration from 0.4 to 10 μg L−1. A diabetic animal model (diabetic zebrafish model) was then applied to investigate 5-HT as a novel biomarker of diabetes. A limit of detection (LOD) of about 0.33 μg L−1 was found for the diabetic group and 0.15 μg L−1 for the control group. The average levels of 5-HT obtained were 9 and 2 μg L−1 for control and diabetic groups, respectively. The recovery, relative standard deviation (RSD), and relative error (RE) were found to be about 97%, less than 2%, and around 3%, respectively. The significant reduction in 5-HT level in the diabetic group compared to the control group proved that the biomarker 5-HT can be applied for the early diagnosis of diabetes mellitus. Graphical abstract: [Figure not available: see fulltext.] © 2021, Springer-Verlag GmbH Germany, part of Springer Nature.

2. Design and Fabrication of Electrochemical Biosensor Based on Nano-Graphene to Detect Serotonin in Diabetic Zebra Fish, Iranian Journal of Diabetes and Metabolism (2020)

3. Nanomaterial Based Electrochemical Sensing of the Biomarker Serotonin: A Comprehensive Review, Microchimica Acta (2019)

4. Tryptophan and Serotonin Levels As Potent Biomarkers in Diabetes Mellitus Complications: A New Approach of Diagnostic Role, Journal of Diabetes and Metabolic Disorders (2022)

5. Electrochemical Detection of Serotonin: A New Approach, Clinica Chimica Acta (2020)

6. Interactive Relationship Between Trp Metabolites and Gut Microbiota: The Impact on Human Pathology of Disease, Journal of Applied Microbiology (2022)

7. Visualization of Knowledge Flow in Interpersonal Scientific Collaboration Network Endocrinology and Metabolism Research Institute, Journal of Diabetes and Metabolic Disorders (2021)

8. Concurrent Metformin and Silibinin Therapy in Diabetes: Assessments in Zebrafish (Danio Rerio) Animal Model, Journal of Diabetes and Metabolic Disorders (2020)

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Mohammad Reza Khorramizadeh (6)

Bagher Larijani (6)

Style	Citing Format
MLA	Khoshnevisan K, et al.. "Serotonin Level As a Potent Diabetes Biomarker Based on Electrochemical Sensing: A New Approach in a Zebra Fish Model." Analytical and Bioanalytical Chemistry, vol. 413, no. 6, 2021, pp. 1615-1627.
APA	Khoshnevisan K, Baharifar H, Torabi F, Sadeghi Afjeh M, Maleki H, Honarvarfard E, Mohammadi H, Sajjadijazi SM, Mahmoudikohan S, Faridbod F, Larijani B, Saadat F, Faridi Majidi R, Khorramizadeh MR (2021). Serotonin Level As a Potent Diabetes Biomarker Based on Electrochemical Sensing: A New Approach in a Zebra Fish Model. Analytical and Bioanalytical Chemistry, 413(6), 1615-1627.
Chicago	Khoshnevisan K, Baharifar H, Torabi F, Sadeghi Afjeh M, Maleki H, Honarvarfard E, Mohammadi H, et al.. "Serotonin Level As a Potent Diabetes Biomarker Based on Electrochemical Sensing: A New Approach in a Zebra Fish Model." Analytical and Bioanalytical Chemistry 413, no. 6 (2021): 1615-1627.
Harvard	Khoshnevisan K et al. (2021) 'Serotonin Level As a Potent Diabetes Biomarker Based on Electrochemical Sensing: A New Approach in a Zebra Fish Model', Analytical and Bioanalytical Chemistry, 413(6), pp. 1615-1627.
Vancouver	Khoshnevisan K, Baharifar H, Torabi F, Sadeghi Afjeh M, Maleki H, Honarvarfard E, et al.. Serotonin Level As a Potent Diabetes Biomarker Based on Electrochemical Sensing: A New Approach in a Zebra Fish Model. Analytical and Bioanalytical Chemistry. 2021;413(6):1615-1627.
BibTex	@article{ author = {Khoshnevisan K and Baharifar H and Torabi F and Sadeghi Afjeh M and Maleki H and Honarvarfard E and Mohammadi H and Sajjadijazi SM and Mahmoudikohan S and Faridbod F and Larijani B and Saadat F and Faridi Majidi R and Khorramizadeh MR}, title = {Serotonin Level As a Potent Diabetes Biomarker Based on Electrochemical Sensing: A New Approach in a Zebra Fish Model}, journal = {Analytical and Bioanalytical Chemistry}, volume = {413}, number = {6}, pages = {1615-1627}, year = {2021} }
RIS	TY - JOUR AU - Khoshnevisan K AU - Baharifar H AU - Torabi F AU - Sadeghi Afjeh M AU - Maleki H AU - Honarvarfard E AU - Mohammadi H AU - Sajjadijazi SM AU - Mahmoudikohan S AU - Faridbod F AU - Larijani B AU - Saadat F AU - Faridi Majidi R AU - Khorramizadeh MR TI - Serotonin Level As a Potent Diabetes Biomarker Based on Electrochemical Sensing: A New Approach in a Zebra Fish Model JO - Analytical and Bioanalytical Chemistry VL - 413 IS - 6 SP - 1615 EP - 1627 PY - 2021 ER -

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