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Phylogeography and Genetic Diversity of Human Hydatidosis in Bordering the Caspian Sea, Northern Iran by Focusing on Echinococcus Granulosus Sensu Stricto Complex



Siyadatpanah A1, 2 ; Daryani A3 ; Sarvi S3 ; Spotin A4 ; Sharif M3 ; Esmaeelzadeh Dizaji R5 ; Anvari D3 ; Emami Zeydi A6 ; Kohansal MH7 ; Agudelo Higuita NI8 ; Hosseini SA3 ; Aryaeipour M9 ; Gholami S3
Authors
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Authors Affiliations
  1. 1. Ferdows School of Paramedical and Health, Birjand University of Medical Sciences, Birjand, Iran
  2. 2. Mazandaran University of Medical Sciences, Sari, Iran
  3. 3. Department of Parasitology, Toxoplasmosis Research Center, Mazandaran University of Medical Sciences, Sari, Iran
  4. 4. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
  5. 5. Department of Poultry Disease, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
  6. 6. Department of Medical-Surgical Nursing, Nasibeh School of Nursing and Midwifery, Mazandaran University of Medical Sciences, Sari, Iran
  7. 7. School of Medicine, Bam University of Medical Sciences, Bam, Iran
  8. 8. Division of Infectious Diseases Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK, United States
  9. 9. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Source: Iranian Journal of Public Health Published:2020

Abstract

Background: Human Echinococcosisis a cyclo-zoonotic infection caused by tapeworms of the Echinococcus granulosus sensu stricto complex. The detection of mitochondrial genome data of genus Echinococcus can reflect the taxonomic status, genetic diversity, and population structure genetics. Methods: Totally, 52 formalin-fixed paraffin-embedded (FFPE) tissue samples from patients with histologically confirmed CE were collected from Mazandaran province, Iran in the period of Mar 1995 to May 2018. All extracted DNAs from (FFPE) tissue samples were subjected to amplify by polymerase chain reactions method targeting cytochrome c oxidase subunit 1 (cox1) gene. All PCR amplicons were sequenced to phylogenetic analysis and genetic diversity. Results: Molecular analysis showed that 50(96.1%) and 2 (3.84%) isolates were identified as G1 andG3 E. granulosus genotypes, respectively. DNA sequence analyses indicated a high gene diversity for G1 (Haplotype diversity: 0.830) and G3 genotypes (Hd: 1.00). Based on multiple sequence alignment analyses, 7 (13.46%; G1 genotype) and 2 (3.84%; G3 genotype) new haplotypes were unequivocally identified. Conclusion: G3 genotype (Buffalo strain) was identified from two human hydatidosis isolates in the region. Present study strengthens our knowledge about taxonomic status, transmission patterns of Echinococcus parasite to human and heterogeneity aspects of this parasite in clinical CE isolates of Northern Iran. © 2020, Iranian Journal of Public Health. All rights reserved.
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