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Genetic Characterization of Echinococcus Granulosus Sensu Lato in Livestock and Human Isolates From North of Iran Indicates the Presence of E. Ortleppi in Cattle Publisher Pubmed



Nematdoost K1, 2 ; Ashrafi K2 ; Majidishad B2 ; Kia EB3 ; Zeinali A4 ; Sharifdini M1, 2
Authors
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Authors Affiliations
  1. 1. Cellular and Molecular Research Center, Guilan University of Medical Sciences, Rasht, Iran
  2. 2. Department of Medical Parasitology and Mycology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
  3. 3. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Veterinary Head Office of Guilan Province, Rasht, Iran

Source: Acta Parasitologica Published:2021


Abstract

Purpose: Identification of different genotypes of echinococcal cyst in various domestic herbivores and humans within the target area was the principal aim of the present study, performed using sequence data of cox1 and nad1 mitochondrial genes. Methods: A total of 57 cystic echinococcosis (CE) cysts were isolated from indigenous livestock including 45 cattle, 9 sheep and 3 goats from several slaughterhouses in Guilan Province. Moreover, 12 formalin-fixed paraffin-embedded (FFPE) CE cyst tissues from humans were also included, obtained from the archives of several hospitals in Rasht, the capital of Guilan. Genetic sequencing was conducted using mitochondrial cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit 1 (nad1) genes. Results: Our results found that E. granulosus sensu stricto (s.s.) and E. ortleppi were present in 92.7% and 7.2% isolates, respectively. E. granulosus s.s. (genotypes G1 and G3) and E. ortleppi were isolated from various livestock whereas all CE cysts isolated from humans were E. granulosus s.s. G1 genotype. Conclusion: We found that E. granulosus s.s. G1 was the predominant genotype within the study region. This is the first study to report E. ortleppi in cattle in Iran. © 2020, Witold Stefanski Institute of Parasitology, Polish Academy of Sciences.
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