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Histopathologic Alterations Between Echinococcus Granulosus Sensu Stricto and E. Canadensis Genotypes of Human Cystic Echinococcosis Cysts in Shiraz, Iran Publisher



Darabi E1, 2 ; Sadjjadi SM1 ; Mohammadzadeh T3 ; Karamian M4 ; Owji SM5 ; Sedaghat B1
Authors
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Authors Affiliations
  1. 1. Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
  2. 2. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Applied Microbiology Research Center, Systems Biology and Poisoning Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
  4. 4. Department of Parasitology and Mycology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
  5. 5. Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

Source: Iranian Journal of Parasitology Published:2025


Abstract

Background: We aimed to determine the genotypes of Echinococcus granulosus sensu lato (s.l.) using DNA regions within the NADH dehydrogenase subunit-1 (nad1) mitochondrial genes in formalin-fixed paraffin-embedded (FFPE) isolates of human cystic echinococcosis cysts (CE cysts) and compare their histopathologic alterations. Methods: Out of 135 samples, 21 high-quality PCR positive samples were selected for sequencing and were deposited into GenBank database. Moreover, histopathological changes of E. granulosus sensu stricto (G1 genotype) and E. canadensis (G6 genotype) cases were also compared. Results: Based on the sequencing results, 16 cases were diagnosed as E. granulosus s.s. (G1-G3 genotype) and 5 cases as E. canadensis (G6 genotype). Five haplotypes of E. granulosus were identified from 21 nad1 sequences. The histopathological alterations in both genotypes showed laminated layer of CE without inflammatory cells. In a few cases of the G6 geno-type, neutrophils in the outer cuticular layer with mild vascular and congestion were observed. Cell debris with multiple areas of necrosis, as well as scanty lymphoplasma cells in the outer cuticular layer were observed in G1-G3 genotype cases. So, the histopathological differences between the two genotypes are not noticeable enough to be differentiated by microscopical observations. Conclusion: E. granulosus s.s. (G1-G3) and E. canadensis (G6 genotype) are prevalent among CE patients. In general, five haplotypes were identified by nad1 genes analysis. The histopathological differences between the two genotypes have not been so big to be differentiated by microscopic observations. © 2025 Darabi et al. Published by Tehran University of Medical Sciences.
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