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Differential Effect of Activin a and Wnt3a on Definitive Endoderm Differentiation on Electrospun Nanofibrous Pcl Scaffold Publisher Pubmed



Hoveizi E1 ; Massumi M2 ; Ebrahimibarough S3, 4 ; Tavakol S5, 6, 7 ; Ai J3, 4
Authors
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Authors Affiliations
  1. 1. Department of Biology, Faculty of Sciences, Shahid Chamran University of Ahvaz, Ahvaz, Iran
  2. 2. Department of Physiology, University of Toronto, Toronto, Canada
  3. 3. Department of Tissue Engineering and Applied Cell Sciences, Faculty of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Brain and Spinal Cord Injury Research Center (BASIR), Tehran University of Medical Sciences, Keshavarz Boulevard, Gharib Street, Tehran, 6114185, Iran
  5. 5. Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran
  6. 6. Advanced Medical Science and Technologies Association, Student's Scientific Research Center, Tehran University of Medical Sciences, Tehran, Iran
  7. 7. Nanopharmaceutics Laboratory, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran

Source: Cell Biology International Published:2015


Abstract

The first step in the formation of hepatocytes and beta cells is the generation of definitive endoderm (DE) which involves a central issue in developmental biology. Human induced pluripotent stem cells (hiPSCs) have the pluripotency to differentiate into all three germ layers in vitro and have been considered potent candidates for regenerative medicine as an unlimited source of cells for therapeutic applications. In this study, we investigated the differentiating potential of hiPSCs on poly (ε-caprolactone) (PCL) nanofibrous scaffold into DE cells. Here, we demonstrate directed differentiation of hiPSCs by factors such as Activin A and Wnt3a. The differentiation was determined by immunofluoresence staining with Sox17, FoxA2 and Goosecoid (Gsc) and also by qRT-PCR analysis. The results of this study showed that hiPSCs, as a new cell source, have the ability to differentiate into DE cells with a high capacity and also demonstrate that three dimension (3D) culture provides a suitable nanoenviroment for growth, proliferation and differentiation of hiPSCs. PCL nanofibrous scaffold with essential supplements, stimulating factors and EB-derived cells is able to provide a novel method for enhancing functional differentiation of hiPSCs into DE cells. © 2015 International Federation for Cell Biology.
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