Magnetic Carbon–Based Nanocomposite Decorated With Palladium Complex for Co-Delivery of Dox/Pcrispr Publisher



Bagherzadeh M¹ ; Safarkhani M¹ ; Daneshgar H¹ ; Radmanesh F^{2, 3} ; Taghavimandi F¹ ; Ghadiri AM¹ ; Kiani M¹ ; Fatahi Y^{4, 5, 6} ; Safarialighiarloo N⁷ ; Ahmadi S⁸ ; Rabiee N^{9, 10, 11} Show Affiliations
Source: Journal of Drug Delivery Science and Technology Published:2022

Abstract

Hybrid inorganic/organic compounds opened up the window of opportunities to a wide variety of fields. The carbon-based nanocomposite (Fe3O4/MWCNT-COOH/Extract/PdL@DOX/p-CRISPR/Extract) have been prepared using tangerine and egg white extract as a second layer of nanocomposites. Then, doxorubicin (DOX) and PdC16H10N4O3 (PdL) were added to the mixture. Based on the previous studies, L (carboxamide-based ligand) has a potent desire for connecting and then blocking the HER-2, and σ2 (tumor's overexpressed receptors) and PdL could enhance the sustainability of the DOX by hydrogen bonding and π-π interaction. The effect of biotin on site-specific delivery has been investigated by utilizing two different types of extracts that naturally has a different amount of biotin. Based on this study's results, settling natural extracts (tangerine and egg white), bioactive complexes, and Fe3O4 on the functionalized-MWCNT can strengthen the nanocarrier's stability in the biological matrix, make more active porosity, and control the internalization to the cells for drug/gene delivery. The nanocarrier has revealed 29.1% ± 1.5% GFP positive transfection efficiency. Also, MTT assay (HEK-293, MCF-7 and PC-12) and antibacterial activity and in vivo testing (H&E and AST:ALT analysis) have been screened and showed promising results. © 2022