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Multifunctional 3D Hierarchical Bioactive Green Carbon-Based Nanocomposites Publisher



Rabiee N1 ; Bagherzadeh M1 ; Ghadiri AM1 ; Kiani M1 ; Fatahi Y2, 3, 4 ; Tavakolizadeh M5 ; Pourjavadi A5 ; Jouyandeh M6 ; Saeb MR7 ; Mozafari M8 ; Shokouhimehr M9 ; Varma RS10
Authors
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Authors Affiliations
  1. 1. Department of Chemistry, Sharif University of Technology, Tehran, 11155-3516, Iran
  2. 2. Department of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, 14155-6451, Iran
  3. 3. Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, 14155-6451, Iran
  4. 4. Universal Scientific Education and Research Network (USERN), Tehran, 15875-4413, Iran
  5. 5. Polymer Research Laboratory, Department of Chemistry, Sharif University of Technology, Tehran, 11155-3516, Iran
  6. 6. Center of Excellence in Electrochemistry, School of Chemistry, College of Science, University of Tehran, Tehran, 1417466191, Iran
  7. 7. LMOPS, Universite de Lorraine, CentraleSupelec, Metz, F-57000, France
  8. 8. Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, University of Toronto, Toronto, M5G 1X5, ON, Canada
  9. 9. Department of Materials Science and Engineering, Research Institute of Advanced Materials, Seoul National University, Seoul, 08826, South Korea
  10. 10. Regional Centre of Advanced Technologies and Materials, Palacky University, Slechtitelu 27, Olomouc, 783 71, Czech Republic

Source: ACS Sustainable Chemistry and Engineering Published:2021


Abstract

Carbon-based nanocarriers such as multiwall carbon nanotubes (MWCNTs) and reduced graphene oxide (rGO) have shown promising delivery capabilities due to their low immunogenicity, superior internalization, and suitable cell penetration efficiency. Herein, a molecular engineering strategy is advanced for the one-pot synthesized rGO/MWCNT/Fe3O4/ZnO to enhance the stability of the nanocarrier in the biological matrix; green synthesized ZnO was responsible for water uptake and reduced cytotoxicity, while Fe3O4controlled the cellular internalization for gene delivery. Surface morphology of the ensuing nanocomposite was correlated with photocatalytic and gene delivery (CRISPR/Cas9) features. For the first time, a complete physical interaction between CRISPR/Cas9 and nanomaterial is evidenced via atomic force microscopy (AFM), demonstrating an increase in green fluorescence protein (EGFP) up to 11%. Furthermore, the enhanced photocatalytic activity is displayed in complete degradation of the methylene blue dye under 10 min with an efficiency of over 98%. The cytotoxicity of the nanocomposite is enhanced by ZnO on treatment with the PC12 and HEK-293 cell lines subsequent to 24, 48, and 72 h of exposure, with more than 88, 79, and 80% cell viabilities for PC12 and more than 88, 80, and 85% cell viabilities for HEK-293 in the maximum ratio of material to CRISPR (WRof nM/CC being 100). Furthermore, the nanocomposite showed an antibacterial activity against bothStaphylococcus aureusandEscherichia colibacteria (MZI values of 24 and 21 mm, respectively). The surface chemistry of the optimized system opens up new prospects to codeliver therapeutic agents for useful clinical applications. © 2021 American Chemical Society
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