Combination of Metformin and Chlorogenic Acid Attenuates Hepatic Steatosis and Inflammation in High-Fat Diet Fed Mice

Combination of Metformin and Chlorogenic Acid Attenuates Hepatic Steatosis and Inflammation in High-Fat Diet Fed Mice Publisher Pubmed

Zamanigarmsiri F¹ ; Ghasempour G² ; Aliabadi M¹ ; Hashemnia SMR¹ ; Emamgholipour S¹ ; Meshkani R¹

Show Affiliations

1. Department of Clinical Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Clinical Biochemistry, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran

Source: IUBMB Life Published:2021

Abstract

Non-alcoholic fatty liver disease (NAFLD) has become an important health problem in the world. Natural products, with anti-inflammatory properties, are potential candidates for alleviating NAFLD. Metformin (MET) and chlorogenic acid (CGA) have been reported to be effective in the improvement of NAFLD. Here, we aimed to evaluate the efficacy of MET and CGA combination in ameliorating NAFLD in high-fat diet (HFD) fed mice. Fifty C57BL/6 male mice were divided into two groups, one fed a standard chow diet (n = 10) and the other was fed an HFD (n = 40) for 10 weeks. Animals in the HFD group were then randomly divided into a four groups (HFD, HFD + MET (0.25%), HFD + CGA (0.02%) and HFD + MET + CGA (0.25 + 0.02%). MET and CGA combination decreases fasting blood glucose and improves glucose intolerance. Decreased hepatic triglyceride level was associated with lower expression levels of fatty acid synthase and sterol regulatory element-binding protein-1c in MET+CGA treated mice. MET and CGA combination treatment resulted in the polarization of macrophages to the M2 phenotype, reduction of the expression of pro-inflammatory cytokines (TNF-α, IL-1β and IL-6), and decreasing protein level of NF-kB p65. It was found that the lowering effect of combined MET and CGA on the expression of gluconeogenic genes was accompanied by increasing phosphorylation of glycogen synthase kinase 3β. Treatment of HFD mice with the combination of MET and CGA was found to be more effective at alleviating inflammation and lipid accumulation by increasing phosphorylation of AMP-activated protein kinase. In conclusion, these findings suggest that the MET + CGA combination might exert therapeutic effects against NAFLD. © 2020 International Union of Biochemistry and Molecular Biology

Related Docs

View other Related Docs

1. Combination of Metformin and Genistein Alleviates Non-Alcoholic Fatty Liver Disease in High-Fat Diet-Fed Mice, Journal of Nutritional Biochemistry (2021)

2. Metformin-Chlorogenic Acid Combination Reduces Skeletal Muscle Inflammation in C57bl/6 Mice on High-Fat Diets, Molecular Biology Reports (2023)

3. The Effect of Green Coffee Extract Supplementation on Cardio Metabolic Risk Factors: A Systematic Review and Meta-Analysis of Randomized Controlled Trials, Journal of Diabetes and Metabolic Disorders (2020)

4. Metformin in Combination With Genistein Ameliorates Skeletal Muscle Inflammation in High-Fat Diet Fed C57bl/6 Mice, Cytokine (2021)

5. A Comprehensive Systematic Review of the Effects of Naringenin, a Citrus-Derived Flavonoid, on Risk Factors for Nonalcoholic Fatty Liver Disease, Advances in Nutrition (2021)

6. Combinational Administration of Mesenchymal Stem Cell-Derived Exosomes and Metformin Reduces Inflammatory Responses in an in Vitro Model of Insulin Resistance in Hepg2 Cells, Heliyon (2023)

7. Inhibiting Mir-27A and Mir-142-5P Attenuate Nonalcoholic Fatty Liver Disease by Regulating Nrf2 Signaling Pathway, IUBMB Life (2020)

8. Combination Therapy of Metformin and Morin Attenuates Insulin Resistance, Inflammation, and Oxidative Stress in Skeletal Muscle of High-Fat Diet-Fed Mice, Phytotherapy Research (2024)

Experts (# of related papers)

View all Related Experts

Reza Meshkani (8)

Ghodratollah Panahi (3)

Style	Citing Format
MLA	Zamanigarmsiri F, et al.. "Combination of Metformin and Chlorogenic Acid Attenuates Hepatic Steatosis and Inflammation in High-Fat Diet Fed Mice." IUBMB Life, vol. 73, no. 1, 2021, pp. 252-263.
APA	Zamanigarmsiri F, Ghasempour G, Aliabadi M, Hashemnia SMR, Emamgholipour S, Meshkani R (2021). Combination of Metformin and Chlorogenic Acid Attenuates Hepatic Steatosis and Inflammation in High-Fat Diet Fed Mice. IUBMB Life, 73(1), 252-263.
Chicago	Zamanigarmsiri F, Ghasempour G, Aliabadi M, Hashemnia SMR, Emamgholipour S, Meshkani R. "Combination of Metformin and Chlorogenic Acid Attenuates Hepatic Steatosis and Inflammation in High-Fat Diet Fed Mice." IUBMB Life 73, no. 1 (2021): 252-263.
Harvard	Zamanigarmsiri F et al. (2021) 'Combination of Metformin and Chlorogenic Acid Attenuates Hepatic Steatosis and Inflammation in High-Fat Diet Fed Mice', IUBMB Life, 73(1), pp. 252-263.
Vancouver	Zamanigarmsiri F, Ghasempour G, Aliabadi M, Hashemnia SMR, Emamgholipour S, Meshkani R. Combination of Metformin and Chlorogenic Acid Attenuates Hepatic Steatosis and Inflammation in High-Fat Diet Fed Mice. IUBMB Life. 2021;73(1):252-263.
BibTex	@article{ author = {Zamanigarmsiri F and Ghasempour G and Aliabadi M and Hashemnia SMR and Emamgholipour S and Meshkani R}, title = {Combination of Metformin and Chlorogenic Acid Attenuates Hepatic Steatosis and Inflammation in High-Fat Diet Fed Mice}, journal = {IUBMB Life}, volume = {73}, number = {1}, pages = {252-263}, year = {2021} }
RIS	TY - JOUR AU - Zamanigarmsiri F AU - Ghasempour G AU - Aliabadi M AU - Hashemnia SMR AU - Emamgholipour S AU - Meshkani R TI - Combination of Metformin and Chlorogenic Acid Attenuates Hepatic Steatosis and Inflammation in High-Fat Diet Fed Mice JO - IUBMB Life VL - 73 IS - 1 SP - 252 EP - 263 PY - 2021 ER -

Science Communicator Platform

Authors

Authors Affiliations

Abstract