Mir-21 Expression in Wilms’ Tumor

Mir-21 Expression in Wilms’ Tumor

Bazzaz N¹ ; Nouraee N^{2, 3} ; Zaremirzaie A⁴ ; Shahali M⁵ ; Mowla SJ⁵ ; Vasei M⁶

Show Affiliations

1. Dept. of Pathology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
2. Dept. of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
3. Dept. of Experimental Therapeutics, MD Anderson Cancer Center, University of Texas, Houston, TX, United States
4. Dept. of Molecular Pathology and Cytogenetic, Tehran University of Medical Sciences, Tehran, Iran
5. Dept. of Quality Control, Pasteur Institute of Iran, Tehran, Iran
6. Cell Based Therapies Research Center, Digestive Disease Research Institute, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran

Source: Iranian Journal of Pathology Published:2018

Abstract

Background and Objective: Wilms’ tumor (WT) is the most common genitourinary tract tumor in children. MicroRNAs (miRNAs) are small non-coding RNAs; their role in the pathogenesis of many types of human cancers has been identified. We aimed to evaluate the expression of miR-21, a well-known oncomir, in WT tissue samples which is a very common urinary tract malignancy in children. Methods: We performed chromogenic in situ hybridization (CISH) to detect the sub-cellular localization of miR-21 in 25 formalin-fixed, paraffin-embedded (FFPE) samples of WT. We also evaluated miR-21 expression in 24 of these blocks and 6 normal kidneys as controls using quantitative real-time PCR technique. Results: While our real-time PCR analysis showed miR-21 significant overexpression in 4 tumors compared to the normal kidney samples, we could not detect significant ISH signal in any of these samples. Conclusion: Low expression of miR-21 in WT might pinpoint the weak involvement of this miRNA in the pathogenesis of this cancer. © 2018, IRANIAN JOURNAL OF PATHOLOGY.

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Style	Citing Format
MLA	Bazzaz N, et al.. "Mir-21 Expression in Wilms’ Tumor." Iranian Journal of Pathology, vol. 13, no. 4, 2018, pp. 422-428.
APA	Bazzaz N, Nouraee N, Zaremirzaie A, Shahali M, Mowla SJ, Vasei M (2018). Mir-21 Expression in Wilms’ Tumor. Iranian Journal of Pathology, 13(4), 422-428.
Chicago	Bazzaz N, Nouraee N, Zaremirzaie A, Shahali M, Mowla SJ, Vasei M. "Mir-21 Expression in Wilms’ Tumor." Iranian Journal of Pathology 13, no. 4 (2018): 422-428.
Harvard	Bazzaz N et al. (2018) 'Mir-21 Expression in Wilms’ Tumor', Iranian Journal of Pathology, 13(4), pp. 422-428.
Vancouver	Bazzaz N, Nouraee N, Zaremirzaie A, Shahali M, Mowla SJ, Vasei M. Mir-21 Expression in Wilms’ Tumor. Iranian Journal of Pathology. 2018;13(4):422-428.
BibTex	@article{ author = {Bazzaz N and Nouraee N and Zaremirzaie A and Shahali M and Mowla SJ and Vasei M}, title = {Mir-21 Expression in Wilms’ Tumor}, journal = {Iranian Journal of Pathology}, volume = {13}, number = {4}, pages = {422-428}, year = {2018} }
RIS	TY - JOUR AU - Bazzaz N AU - Nouraee N AU - Zaremirzaie A AU - Shahali M AU - Mowla SJ AU - Vasei M TI - Mir-21 Expression in Wilms’ Tumor JO - Iranian Journal of Pathology VL - 13 IS - 4 SP - 422 EP - 428 PY - 2018 ER -

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