Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Altered Mir-223 Expression in Sputum for Diagnosis of Non-Small Cell Lung Cancer



Bagheri A1 ; Khorshid HRK1 ; Mowla SJ2 ; Mohebbi HA3 ; Mohammadian A4 ; Yaseri M5 ; Solaymanidodaran M6 ; Sherafatian M2 ; Tavallaie M7
Authors
Show Affiliations
Authors Affiliations
  1. 1. Genetic Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran
  2. 2. Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
  3. 3. Trauma Research Center, Baghiyatallah University of Medical Sciences, Tehran, Iran
  4. 4. Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
  5. 5. Department of Epidemiology and Biostatistics, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Iran University of Medical Sciences, Tehran, Iran
  7. 7. Human Genetic Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

Source: Avicenna Journal of Medical Biotechnology Published:2017

Abstract

Background: Diagnosis of Non-small Cell Lung Cancer (NSCLC) at an early stage is a daunting challenge due to the deficiency of specific noninvasive markers. MicroRNAs (miRNAs) play important roles in the initiation and progression of NSCLC. Measuring miRNA expression levels could provide a potential approach for the diagnosis of NSCLC. Our goals were to examine miR-223, miR-212, miR-192, miR-3074, SNORD33 and SNORD37 expression levels in tissue and sputum of NSCLC patients and cancer free subjects for molecular diagnosis of NSCLC. Methods: Relative expressions of miR-223, miR-212, miR-192, miR-3074, SNORD33 and SNORD37 were examined with quantitative real-time RT-PCR assay in tissue and sputum obtained from 17 NSCLC patients and 17 controls. Results: miR-3074 was upregulated in tissue samples of NSCLC patients compared with control group. miR-223 was upregulated, miR-212 and SNORD37 were downergulated in sputum samples of patients compared with controls. miR-223 quantification produced 82% sensitivity and 95% specificity with areas under the ROC curve at 0.90 in detection of NSCLC. Conclusion: miR-223 clearly discriminated cancer patients from cancer-free subjects and our results suggest that miR-223 could be a diagnostic useful biomarker. The measurement of altered miRNA expression in sputum samples manifested the potential noninvasive approach for detection of lung cancer. © 2017, Avicenna Journal of Medical Biotechnology. All rights reserved.
Related Docs
View other Related Docs
1. Cancer Genetics at a Glance: The Comprehensive Insights, Cancer Genetics and Psychotherapy (2017)
2. Exosomes and Micrornas in Biomedical Science, Synthesis Lectures on Biomedical Engineering (2022)
3. Circulating Micrornas in Hepatocellular Carcinoma: Potential Diagnostic and Prognostic Biomarkers, Current Pharmaceutical Design (2016)
4. Microrna-Based Linkage Between Aging and Cancer: From Epigenetics View Point, Cell Journal (2016)
5. How Micrornas Affect the Pd-L1 and Its Synthetic Pathway in Cancer, International Immunopharmacology (2020)
6. Effects of Micro Rnas and Their Targets in Periodontal Diseases, Infectious Disorders - Drug Targets (2018)
7. Increased Levels of Serum and Tissue Mir-107 in Human Gastric Cancer: Correlation With Tumor Hypoxia, Cancer Biomarkers (2015)
8. Downregulation of Plasma Mir-142-3P and Mir-26A-5P in Patients With Colorectal Carcinoma, International Journal of Cancer Management (2015)
Experts (# of related papers)
View all Related Experts
Hamid Reza Mirzaei (4)
Reza Malekzadeh (1)
Other Related Docs
9. Lung Cancer and Mirnas: A Possible Remedy for Anti-Metastatic, Therapeutic and Diagnostic Applications, Expert Review of Respiratory Medicine (2017)
10. Circulating Micrornas As Diagnostic and Therapeutic Biomarkers in Gastric and Esophageal Cancers, Journal of Cellular Physiology (2018)
11. Epigenetic Regulation in Myocardial Infarction: Non-Coding Rnas and Exosomal Non-Coding Rnas, Frontiers in Cardiovascular Medicine (2022)
12. A Universal Ratiometric Method for Micro-Rna Detection Based on the Ratio of Electrochemical/Electrochemiluminescence Signal, and Toehold-Mediated Strand Displacement Amplification, Analytica Chimica Acta (2023)
13. A Preliminary Study of Microrna-208B After Acute Myocardial Infarction: Impact on 6-Month Survival, Disease Markers (2018)
14. The Role of Oncomirs in the Pathogenesis and Treatment of Breast Cancer, Biomedicine and Pharmacotherapy (2016)
15. Gynecologic Cancers and Non-Coding Rnas: Epigenetic Regulators With Emerging Roles, Critical Reviews in Oncology/Hematology (2021)
16. Extracellular Microrna Profiling in Human Follicular Fluid: New Biomarkers in Female Reproductive Potential, Journal of Assisted Reproduction and Genetics (2020)
17. Biomarker Potential of Competing Endogenous Rna Networks in Polycystic Ovary Syndrome (Pcos), Non-coding RNA Research (2024)
18. Liquid Biopsy in Male Genital Tract: Testis Tumor, Liquid Biopsy in Urogenital Cancers and its Clinical Utility (2022)
19. An Innovative Paradigm of Methods in Micrornas Detection: Highlighting Dnazymes, the Illuminators, Biosensors and Bioelectronics (2018)
20. Plasma Mir-183-5P in Colorectal Cancer Patients As Potential Predictive Lymph Node Metastasis Marker, Journal of Cancer Research and Therapeutics (2022)
21. Microrna As a Systemic Intervention in the Specific Breast Cancer Subtypes With C-Myc Impacts; Introducing Subtype-Based Appraisal Tool, Journal of Cellular Physiology (2018)
22. Evaluation of Mir-122 Level in the Plasma of Chronically Hcv Infected Patients, Molecular Biology (2016)
23. Liquid Biopsy in Kidney Tumor, Liquid Biopsy in Urogenital Cancers and its Clinical Utility (2022)
24. The Effects of All Trans Retinoic Acid, Vitamin D3 and Their Combination on Plasma Levels of Mirna-125A-5P, Mirna-34A, and Mirna-126 in an Experimental Model of Diabetes, Avicenna Journal of Phytomedicine (2022)
25. Reciprocal Interconnection of Mirnome-Epigenome in Cancer Pathogenesis and Its Therapeutic Potential, Epigenetics Territory and Cancer (2015)
26. Ectopic Expression of Mirna-21 and Mirna-205 in Non-Small Cell Lung Cancer, International Journal of Cancer Management (2019)
27. Circulating Micrornas As Predictive Biomarkers of Coronary Artery Diseases in Type 2 Diabetes Patients, Journal of Clinical Laboratory Analysis (2022)
28. Microrna Let-7 and Viral Infections: Focus on Mechanisms of Action, Cellular and Molecular Biology Letters (2022)
29. The Potential for Circulating Micrornas in the Diagnosis of Myocardial Infarction: A Novel Approach to Disease Diagnosis and Treatment, Current Pharmaceutical Design (2016)
30. The Effects of Low-Level Laser Irradiation on Breast Tumor in Mice and the Expression of Let-7A, Mir-155, Mir-21, Mir125, and Mir376b, Lasers in Medical Science (2016)
31. Serum and Tissue Mirnas: Potential Biomarkers for the Diagnosis of Cervical Cancer, Virology Journal (2019)
32. Altered Expression of Micrornas Following Chronic Allograft Dysfunction With Interstitial Fibrosis and Tubular Atrophy, Iranian Journal of Allergy# Asthma and Immunology (2015)
33. Tumor Immunology, Clinical Immunology (2022)