68 Ga-Radiolabeled Bombesin-Conjugated to Trimethyl Chitosan-Coated Superparamagnetic Nanoparticles for Molecular Imaging: Preparation, Characterization and Biological Evaluation

68 Ga-Radiolabeled Bombesin-Conjugated to Trimethyl Chitosan-Coated Superparamagnetic Nanoparticles for Molecular Imaging: Preparation, Characterization and Biological Evaluation Publisher Pubmed

Hajiramezanali M¹ ; Atyabi F^{2, 3} ; Mosayebnia M¹ ; Akhlaghi M⁴ ; Geramifar P⁴ ; Jalilian AR⁴ ; Mazidi SM⁵ ; Yousefnia H⁶ ; Shahhosseini S⁷ ; Beiki D⁴

Show Affiliations

1. Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
2. Department of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
3. Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
4. Research Center for Nuclear Medicine, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
5. Radiation Application Research School, Nuclear Science and Technology Research Institute (NSTRI), Tehran, Iran
6. Material and Nuclear Fuel Research School, Nuclear Science and Technology Research Institute (NSTRI), Tehran, Iran
7. Department of Radiopharmacy and Pharmaceutical Chemistry, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: International Journal of Nanomedicine Published:2019

Abstract

Introduction: Nowadays, nanoparticles (NPs) have attracted much attention in biomedical imaging due to their unique magnetic and optical characteristics. Superparamagnetic iron oxide nanoparticles (SPIONs) are the prosperous group of NPs with the capability to apply as magnetic resonance imaging (MRI) contrast agents. Radiolabeling of targeted SPIONs with positron emitters can develop dual positron emission tomography (PET)/MRI agents to achieve better diagnosis of clinical conditions. Methods: In this work, N,N,N-trimethyl chitosan (TMC)-coated magnetic nanoparticles (MNPs) conjugated to S-2-(4-isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane tetraacetic acid (DOTA) as a radioisotope chelator and bombesin (BN) as a targeting peptide (DOTA-BN- TMC-MNPs) were prepared and validated using fourier transform infrared (FTIR) spectroscopy, transmission electron microscopy (TEM), thermogravimetric analysis (TGA), vibrating sample magnetometer (VSM), and powder X-ray diffraction (PXRD) tests. Final NPs were radiolabeled with gallium-68 ( 68 Ga) and evaluated in vitro and in vivo as a potential PET/MRI probe for breast cancer (BC) detection. Results: The DOTA-BN-TMC-MNPs with a particle size between 20 and 30 nm were efficiently labeled with 68 Ga (radiochemical purity higher than 98% using thin layer chromatography (TLC)). The radiolabeled NPs showed insignificant toxicity (>74% cell viability) and high affinity (IC 50 =8.79 µg/mL) for the gastrin-releasing peptide (GRP)-avid BC T-47D cells using competitive binding assay against 99m Tc-hydrazinonicotinamide (HYNIC)-gamma-aminobutyric acid (GABA)-BN (7-14). PET and MRI showed visible uptake of NPs by T-47D tumors in xenograft mouse models. Conclusion: 68 Ga-DOTA-BN-TMC-MNPs could be a potential diagnostic probe to detect BC using PET/MRI technique. © 2019 Hajiramezanali et al.

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Style	Citing Format
MLA	Hajiramezanali M, et al.. "68 Ga-Radiolabeled Bombesin-Conjugated to Trimethyl Chitosan-Coated Superparamagnetic Nanoparticles for Molecular Imaging: Preparation, Characterization and Biological Evaluation." International Journal of Nanomedicine, vol. 14, no. , 2019, pp. 2591-2605.
APA	Hajiramezanali M, Atyabi F, Mosayebnia M, Akhlaghi M, Geramifar P, Jalilian AR, Mazidi SM, Yousefnia H, Shahhosseini S, Beiki D (2019). 68 Ga-Radiolabeled Bombesin-Conjugated to Trimethyl Chitosan-Coated Superparamagnetic Nanoparticles for Molecular Imaging: Preparation, Characterization and Biological Evaluation. International Journal of Nanomedicine, 14(), 2591-2605.
Chicago	Hajiramezanali M, Atyabi F, Mosayebnia M, Akhlaghi M, Geramifar P, Jalilian AR, Mazidi SM, Yousefnia H, Shahhosseini S, Beiki D. "68 Ga-Radiolabeled Bombesin-Conjugated to Trimethyl Chitosan-Coated Superparamagnetic Nanoparticles for Molecular Imaging: Preparation, Characterization and Biological Evaluation." International Journal of Nanomedicine 14, no. (2019): 2591-2605.
Harvard	Hajiramezanali M et al. (2019) '68 Ga-Radiolabeled Bombesin-Conjugated to Trimethyl Chitosan-Coated Superparamagnetic Nanoparticles for Molecular Imaging: Preparation, Characterization and Biological Evaluation', International Journal of Nanomedicine, 14(), pp. 2591-2605.
Vancouver	Hajiramezanali M, Atyabi F, Mosayebnia M, Akhlaghi M, Geramifar P, Jalilian AR, et al.. 68 Ga-Radiolabeled Bombesin-Conjugated to Trimethyl Chitosan-Coated Superparamagnetic Nanoparticles for Molecular Imaging: Preparation, Characterization and Biological Evaluation. International Journal of Nanomedicine. 2019;14():2591-2605.
BibTex	@article{ author = {Hajiramezanali M and Atyabi F and Mosayebnia M and Akhlaghi M and Geramifar P and Jalilian AR and Mazidi SM and Yousefnia H and Shahhosseini S and Beiki D}, title = {68 Ga-Radiolabeled Bombesin-Conjugated to Trimethyl Chitosan-Coated Superparamagnetic Nanoparticles for Molecular Imaging: Preparation, Characterization and Biological Evaluation}, journal = {International Journal of Nanomedicine}, volume = {14}, number = {}, pages = {2591-2605}, year = {2019} }
RIS	TY - JOUR AU - Hajiramezanali M AU - Atyabi F AU - Mosayebnia M AU - Akhlaghi M AU - Geramifar P AU - Jalilian AR AU - Mazidi SM AU - Yousefnia H AU - Shahhosseini S AU - Beiki D TI - 68 Ga-Radiolabeled Bombesin-Conjugated to Trimethyl Chitosan-Coated Superparamagnetic Nanoparticles for Molecular Imaging: Preparation, Characterization and Biological Evaluation JO - International Journal of Nanomedicine VL - 14 IS - SP - 2591 EP - 2605 PY - 2019 ER -

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