The Hydroalcoholic Extract of Matricaria Chamomilla Suppresses Migration and Invasion of Human Breast Cancer Mda-Mb-468 and Mcf-7 Cell Lines

The Hydroalcoholic Extract of Matricaria Chamomilla Suppresses Migration and Invasion of Human Breast Cancer Mda-Mb-468 and Mcf-7 Cell Lines Publisher

Nikseresht M¹ ; Kamali AM² ; Rahimi HR^{3, 4} ; Delaviz H⁵ ; Toori MA⁶ ; Kashani IR⁷ ; Mahmoudi R⁵

Show Affiliations

1. Department of Biochemistry, Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
2. Stud. Research Committee, Faculty of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran
3. Pharmaceutics Research Center, Institute of Neuropharmacology, Iran
4. Department of Toxicology and Pharmacology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran
5. Department of Anatomy and Embryology, Cellular and Molecular Research Center, Faculty of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran
6. Department of Nutrition, Social Determinants of Health Research Center, Faculty of Health, Yasuj University of Medical Sciences, Yasuj, Iran
7. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Pharmacognosy Research Published:2017

Abstract

Background: Matricaria chamomilla is an aromatic plant with antioxidant, anticancer, and anti-inflammatory properties. However, the inhibitory role of M. chamomilla on migration and invasion of human breast cancer cells remains unclear. Objective: This study investigated the methods to evaluate these anticancer mechanisms of M. chamomilla on human breast cancer MCF-7 and MDA-MB-468 cell lines. Materials and Methods: The cells were treated with hydroalcoholic extract of M. chamomilla at different concentrations (50-1300 μg/mL) for 24, 48, and 72 h in a culture medium containing 10% fetal bovine serum. This study quantified the 50% growth inhibition concentrations (IC50) by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay; apoptosis and necrosis through Hoechst 33342/propidium iodide staining; cell proliferation and clone formation by clonogenic assay as well as cellular migration, invasion, and attachment. After 24, 48, and 72 h of treatment, the IC50levels were 992 ± 2.3 μg/mL, 893 ± 5.4 μg/mL, and 785 ± 4.8 μg/mL against MDA-MB-468, respectively, and 1288 ± 5.6 μg/mL, 926 ± 2.5 μg/mL, and 921 ± 3.5 μg/mL, against MCF-7, respectively. Furthermore, increasing the extract concentrations induced cellular apoptosis and necrosis and decreased cellular invasion or migration through 8 μm pores, colonization and attachment in a dose-dependent manner. Results: It indicated time- and dose-dependent anti-invasive and antimigrative or proliferative and antitoxic effects of hydroalcoholic extract of aerial parts of chamomile on breast cancer cells. Conclusion: This study demonstrated an effective plant in preventing or treating breast cancer. © 2017 Pharmacognosy Research | Published by Wolters Kluwer - Medknow.

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Style	Citing Format
MLA	Nikseresht M, et al.. "The Hydroalcoholic Extract of Matricaria Chamomilla Suppresses Migration and Invasion of Human Breast Cancer Mda-Mb-468 and Mcf-7 Cell Lines." Pharmacognosy Research, vol. 9, no. 1, 2017, pp. 87-95.
APA	Nikseresht M, Kamali AM, Rahimi HR, Delaviz H, Toori MA, Kashani IR, Mahmoudi R (2017). The Hydroalcoholic Extract of Matricaria Chamomilla Suppresses Migration and Invasion of Human Breast Cancer Mda-Mb-468 and Mcf-7 Cell Lines. Pharmacognosy Research, 9(1), 87-95.
Chicago	Nikseresht M, Kamali AM, Rahimi HR, Delaviz H, Toori MA, Kashani IR, Mahmoudi R. "The Hydroalcoholic Extract of Matricaria Chamomilla Suppresses Migration and Invasion of Human Breast Cancer Mda-Mb-468 and Mcf-7 Cell Lines." Pharmacognosy Research 9, no. 1 (2017): 87-95.
Harvard	Nikseresht M et al. (2017) 'The Hydroalcoholic Extract of Matricaria Chamomilla Suppresses Migration and Invasion of Human Breast Cancer Mda-Mb-468 and Mcf-7 Cell Lines', Pharmacognosy Research, 9(1), pp. 87-95.
Vancouver	Nikseresht M, Kamali AM, Rahimi HR, Delaviz H, Toori MA, Kashani IR, et al.. The Hydroalcoholic Extract of Matricaria Chamomilla Suppresses Migration and Invasion of Human Breast Cancer Mda-Mb-468 and Mcf-7 Cell Lines. Pharmacognosy Research. 2017;9(1):87-95.
BibTex	@article{ author = {Nikseresht M and Kamali AM and Rahimi HR and Delaviz H and Toori MA and Kashani IR and Mahmoudi R}, title = {The Hydroalcoholic Extract of Matricaria Chamomilla Suppresses Migration and Invasion of Human Breast Cancer Mda-Mb-468 and Mcf-7 Cell Lines}, journal = {Pharmacognosy Research}, volume = {9}, number = {1}, pages = {87-95}, year = {2017} }
RIS	TY - JOUR AU - Nikseresht M AU - Kamali AM AU - Rahimi HR AU - Delaviz H AU - Toori MA AU - Kashani IR AU - Mahmoudi R TI - The Hydroalcoholic Extract of Matricaria Chamomilla Suppresses Migration and Invasion of Human Breast Cancer Mda-Mb-468 and Mcf-7 Cell Lines JO - Pharmacognosy Research VL - 9 IS - 1 SP - 87 EP - 95 PY - 2017 ER -

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