Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Design and Fabrication of Polyvinylidene Fluoride-Graphene Oxide/Gelatine Nanofibrous Scaffold for Cardiac Tissue Engineering Publisher Pubmed



Dorkhani E1 ; Noorafkan Y2 ; Salehi Z1 ; Ghiass MA3 ; Tafti SHA4 ; Heiranitabasi A3 ; Tavafoghi M5
Authors
Show Affiliations
Authors Affiliations
  1. 1. School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran
  2. 2. Department of Chemical Engineering, Isfahan University of Technology, Isfahan, Iran
  3. 3. Department of Cell Therapy and Hematology, Faculty of Medical Sciences, Tarbiat Modarres University, Tehran, Iran
  4. 4. Research Center for Advanced Technologies in Cardiovascular Medicine, Cardiovascular Diseases, Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Bioengineering, University of California-Los Angeles, Los Angeles, CA, United States

Source: Journal of Biomaterials Science# Polymer Edition Published:2023


Abstract

Polyvinylidene fluoride (PVDF) electrospun scaffolds have recently been developed for cardiac tissue engineering applications thanks to their piezoelectricity. However, PVDFs’ hydrophobic nature requires modifications by incorporating natural polymers. In this study, we focussed on the hybrid electrospinning of PVDF and gelatine and the further introduction of graphene oxide nanoparticles to investigate either hydrophilicity or piezoelectricity enhancement and its impact on mouse embryonic cardiomyocytes. The results revealed a nanofibre diameter of 379 ± 73 nm for the PVDF/gelatine/graphene oxide (PVDF-GO-CG) platform, providing excellent tensile strength. Additionally, hydrophilicity was improved by gelatine and GO incorporation compared with pure PVDF. Cellular studies also showed an elongated morphology of cardiomyocytes, similar to the myocardial tissue, as well as high viability and non-toxicity in the PVDF-GO-CG scaffold according to the average survival rate. Furthermore, the expression of connexin 43 and troponin T genes underwent an increment of 41 and 35% in the PVDF-GO-CG compared with the PVDF-CG sample. This study proves the applicability of the PVDF-GO-CG scaffold as an alternative substrate for developing engineered cardiac tissues by providing an environment to re-establish their synchronised communications. © 2022 Informa UK Limited, trading as Taylor & Francis Group.
Related Docs
1. In Vitro and in Vivo Studies of Biaxially Electrospun Poly(Caprolactone)/Gelatin Nanofibers, Reinforced With Cellulose Nanocrystals, for Wound Healing Applications, Cellulose (2020)
2. Three-Dimensional Electrospun Gelatin Scaffold Coseeded With Embryonic Stem Cells and Sertoli Cells: A Promising Substrate for in Vitro Coculture System, Journal of Cellular Biochemistry (2019)
3. Whole-Heart Tissue Engineering and Cardiac Patches: Challenges and Promises, Bioengineering (2023)
4. Recent Advances in Electrospun Protein Fibers/Nanofibers for the Food and Biomedical Applications, Advances in Colloid and Interface Science (2023)
5. Electrospun Sandwich-Structured of Polycaprolactone/Gelatin-Based Nanofibers With Controlled Release of Ceftazidime for Wound Dressing, International Journal of Biological Macromolecules (2023)
Experts (# of related papers)
Abdolmohammad Kajbafzadeh (1)