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Combination Meg3 Lncrna and Ciprofloxacin Dramatically Decreases Cell Migration and Viability As Well As Induces Apoptosis in Gc Cells in Vitro Publisher Pubmed



Najafi D1 ; Siri G2 ; Sadri M3 ; Yazdani O4 ; Esbati R5 ; Karimi P6 ; Keshavarz A7 ; Mehmandaroskuie A8 ; Ilktac M1
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Authors Affiliations
  1. 1. Faculty of Pharmacy, Eastern Mediterranean University, North Cyprus, Famagusta, Turkey
  2. 2. Department of Internal Medicine, Amir Alam Hospital, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Internal Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
  4. 4. Department of Medical Science, School of Medicine, Shahid Beheshti University, Tehran, Iran
  5. 5. Research Center for Social Determinants of Health, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  6. 6. Fars Population-Based Cancer Registry, Shiraz University of Medical Sciences, Shiraz, Iran
  7. 7. Department of Hematology and Blood Banking, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  8. 8. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Source: Biotechnology and Applied Biochemistry Published:2024


Abstract

Gastric cancer (GC) is a prominent cause of cancer-related mortality worldwide. Long noncoding RNA (lncRNA) maternal expression gene3 (MEG3) participates in numerous signaling pathways by targeting the miRNA-mRNA axis. Studies on human tumors have demonstrated that the antibiotic Ciprofloxacin induces cell cycle changes, programmed cell death, and growth suppression. In this study, we transfected MEG3 lncRNA and Ciprofloxacin into the MKN-45 GC cell line. qRT-PCR was employed to evaluate the effects on the specific microRNA and mRNA. The wound healing test, MTT assay, and flow cytometry were used to assess the impact of their administration on cell migration, viability, and apoptosis, respectively. Research showed that miR-147 expression fell even more after MEG3 lncRNA transfection, leading to an increase in B-cell lymphoma 2 (BCL-2) levels. Ciprofloxacin transfection did not significantly affect the axis, except for MEG3, which led to its slight upregulation. MEG3 lncRNA inhibited the migration of MKN-45 cells compared to the control group. When MEG3 lncRNA was coupled with Ciprofloxacin, there was a significant reduction in cell migration compared to untreated groups and controls. MTT assay and flow cytometry demonstrated that MEG3 lncRNA decreased cell viability and triggered apoptosis. Simultaneous administration of MEG3 lncRNA and Ciprofloxacin revealed a significant reduction in cell viability caused by increased apoptosis obtained from MTT or flow cytometry assays. Modulating the miR-147-BCL-2 axis decreases cell migration and survival while promoting cell death. In conclusion, combining MEG3 lncRNA with Ciprofloxacin may be an effective therapeutic approach for GC treatment by influencing the miR-14-BCl-2 axis, resulting in reduced cell viability, migration, and increased apoptosis. © 2024 International Union of Biochemistry and Molecular Biology, Inc.
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