Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Synthesis of Fluorescent Cysteine-Gold Nano-Clusters (Cys-Au-Ncs) and Their Application As Nano-Biosensors for the Determination of Cysteine Publisher



Borghei YS1 ; Hosseini M1, 2 ; Ganjali MR3, 4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran
  2. 2. Medical Biomaterials Research Center, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Center of Excellence in Electrochemistry, Faculty of Chemistry, University of Tehran, Tehran, Iran
  4. 4. Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

Source: Current Nanoscience Published:2017


Abstract

Background: Gold nano-clusters (Au-NCs) are non-toxic bio-markers, with higher potentials as compared to quantum dots, and hence the present work was focused on the synthesis of Au-NCs using Cysteine (Cys) as the reducing and protecting agent. Method: The properties of the resulting Cys-Au-NCs were evaluated through scanning electron microscopy (SEM), as well as FT-IR, fluorescence and UV-Vis spectroscopy. Synthesis of the gold nano-clusters was conducted by adding chloroauric acid solution to fresh Cys solutions under stirring. The resultant was kept under stirring in a water bath at 37 °C overnight. Objectives: Considering the low stability of Cys-CuNCs, we decided to study a new approach for determination of Cys through the synthesis of size-selected Au-NCs in the presence of cysteine as a reducing and stabilizing agent, to acquire nanoclusters with higher stability. Results: The spectroscopic results showed various intensities which were attributed to the size of the Cys-Au-NCs. It was also considered that the enhanced fluorescence intensity of Au-NCs observed parallel to increasing the concentration of cysteine could be used for developing proper detection systems for cysteine in aqueous and blood serum samples. This was achieved in the course of the formation of different Cys-Au-NCs with various sizes. Under optimal conditions, the nano-clusters was used as a nano-biosensor and showed excellent selectivity for Cysteine as opposed to the other amino acids tested and produced linear response in the range of 20 to 200 μM. The detection limit of the system was evaluated to reach as low as 8.4 μM. Conclusion: Cysteine-stabilized Au-NCs were successfully synthesized and were found to be stable in aqueous media even after 6 months. Results of the fluorescence emission of the Cys-Au-NCs was found to be selective towards Cys. The nano-biosensor was also successfully used in serum samples for Cys detection. © 2017 Bentham Science Publishers.
Related Docs
View other Related Docs
1. Fluorescence Based Turn-On Strategy for Determination of Microrna-155 Using Dna-Templated Copper Nanoclusters, Microchimica Acta (2017)
2. Label-Free Fluorescent Detection of Microrna-155 Based on Synthesis of Hairpin Dna-Templated Copper Nanoclusters by Etching (Top-Down Approach), Sensors and Actuators# B: Chemical (2017)
3. Detection of Large Deletion in Human Brca1 Gene in Human Breast Carcinoma Mcf-7 Cells by Using Dna-Silver Nanoclusters, Methods and Applications in Fluorescence (2018)
4. Oxidase-Like Catalytic Activity of Cys-Auncs Upon Visible Light Irradiation and Its Application for Visual Mirna Detection, Sensors and Actuators# B: Chemical (2018)
5. A New Fluorescence Turn-On Nanobiosensor for the Detection of Micro-Rna-21 Based on a Dna-Gold Nanocluster, Methods and Applications in Fluorescence (2017)
6. Visual Detection of Mirna Using Peroxidase-Like Catalytic Activity of Dna-Cuncs and Methylene Blue As Indicator, Clinica Chimica Acta (2018)
7. Novel Fluorometric Assay for Detection of Cysteine As a Reducing Agent and Template in Formation of Copper Nanoclusters, Journal of Fluorescence (2017)
8. Detection of P53 Gene Mutation (Single-Base Mismatch) Using a Fluorescent Silver Nanoclusters, Journal of Fluorescence (2017)
Experts (# of related papers)
Mehdi Khoobi (1)
Other Related Docs
9. A Colorimetric Assay of Dna Methyltransferase Activity Based on Peroxidase Mimicking of Dna Template Ag/Pt Bimetallic Nanoclusters, Analytical and Bioanalytical Chemistry (2018)
10. Photoluminescence Mechanisms of Dual-Emission Fluorescent Silver Nanoclusters Fabricated by Human Hemoglobin Template: From Oxidation- and Aggregation-Induced Emission Enhancement to Targeted Drug Delivery and Cell Imaging, ACS Sustainable Chemistry and Engineering (2018)
11. Fluorometric Determination of Microrna Via Fret Between Silver Nanoclusters and Cdte Quantum Dots, Microchimica Acta (2017)
12. A Colorimetric Paper Sensor for Citrate As Biomarker for Early Stage Detection of Prostate Cancer Based on Peroxidase-Like Activity of Cysteine-Capped Gold Nanoclusters, Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy (2019)
13. Peptide-Protected Metal Nanoclusters, Luminescent Metal Nanoclusters: Synthesis# Characterization# and Applications (2022)
14. Dna Methyltransferase Activity Detection Based on Graphene Quantum Dots Using Fluorescence and Fluorescence Anisotropy, Sensors and Actuators# B: Chemical (2017)
15. Environmental Applications of Luminescent Metal Nanoclusters, Luminescent Metal Nanoclusters: Synthesis# Characterization# and Applications (2022)
16. A Fluorometric Study on the Effect of Dna Methylation on Dna Interaction With Graphene Quantum Dots, Methods and Applications in Fluorescence (2019)
17. A Novel Brca1 Gene Deletion Detection in Human Breast Carcinoma Mcf-7 Cells Through Fret Between Quantum Dots and Silver Nanoclusters, Journal of Pharmaceutical and Biomedical Analysis (2018)
18. Colorimetric and Energy Transfer Based Fluorometric Turn-On Method for Determination of Microrna Using Silver Nanoclusters and Gold Nanoparticles, Microchimica Acta (2018)
19. Biomarker Sensing Using Luminescent Metal Nanoclusters, Luminescent Metal Nanoclusters: Synthesis# Characterization# and Applications (2022)
20. A Unique Fret Approach Toward Detection of Single-Base Mismatch Dna in Brca1 Gene, Materials Science and Engineering C (2019)
21. Application of Intercalating Molecules in Detection of Methylated Dna in the Presence of Silver Ions, Methods and Applications in Fluorescence (2019)