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The Effect of Antimicrobial Photodynamic Therapy Using Chlorophyllin-Phycocyanin Mixture on Enterococcus Faecalis: The Influence of Different Light Sources Publisher



Chiniforush N1, 2 ; Pourhajibagher M3 ; Parker S2 ; Benedicenti S2 ; Bahador A4 ; Salagean T5 ; Bordea IR6
Authors
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Authors Affiliations
  1. 1. Dental Implant Research Center, Dentistry Research Institute, Tehran University of Medial Sciences, Tehran, 1441987566, Iran
  2. 2. Department of Surgical Sciences and Integrated Diagnostics, University of Genoa, Genoa, 16132, Italy
  3. 3. Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, 1441987566, Iran
  4. 4. Oral Microbiology Laboratory, Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, 1441987566, Iran
  5. 5. Department of Land Measurements and Exact Sciences, University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, 400372, Romania
  6. 6. Department of Oral Rehabilitation, Faculty of Dentistry, Iuliu Hat, ieganu University of Medicine and Pharmacy, Cluj-Napoca, 400012, Romania

Source: Applied Sciences (Switzerland) Published:2020


Abstract

The purpose of this study was to evaluate the in vitro effect of the chlorophyllin-phycocyanin mixture (Photoactive+) as a photosensitizer (PS) during antimicrobial photodynamic therapy (aPDT) on the count of Enterococcus faecalis (E. faecalis) using different light sources. The antimicrobial effect of aPDT with chlorophyllin-phycocyanin mixture using different light sources including diode laser (λ = 660 nm), diode laser (λ = 635 nm), LED (λ = 450 - 30 nm) alone or in combination was assessed using microbial cell viability assay against E. faecalis. In addition, the cell cytotoxicity of Photoactive+ was assessed on human gingival fibroblast (HuGu) cells by MTT assay; E. faecalis growth when treated by both red wavelengths (635 nm, 660 nm) and combination of LED (420-480 nm) and red wavelengths (635 nm, 660 nm), significantly reduced compared to the control group (p < 0.05). There was no significant reduction in the number of viable cells exposed to Photoactive+ compared to the control group (p < 0.05). This study shows that the application of chlorophyllin-phycocyanin mixture and irradiation with emission of red light achieved a better result for bacterial count reduction, compared to a control. This component can be applied safely due to very negligible cytotoxicity. © 2020 by the authors.
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