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Comparison of Different Modes of Photo-Activated Disinfection Against Porphyromonas Gingivalis: An in Vitro Study Publisher Pubmed



Fekrazad R1, 2 ; Khoei F3 ; Bahador A4 ; Hakimiha N3
Authors
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Authors Affiliations
  1. 1. Radiation Sciences Research Center, Laser Research Center in Medical Sciences, AJA University of Medical Sciences, Tehran, Iran
  2. 2. International Network for Photo Medicine and Photo Dynamic Therapy (INPMPDT), Universal Scientific Education and Research Network (USERN), Tehran, Iran
  3. 3. Laser Research Center of Dentistry, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Oral Microbiology Laboratory, Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Photodiagnosis and Photodynamic Therapy Published:2020


Abstract

Background: Porphyromonas gingivalis (P. gingivalis), a Gram-negative periodontal pathogen, is frequently detected in subgingival plaques in patients with chronic periodontitis. Considering the insufficient results achieved by the current antimicrobial approaches, this study aimed to compare the susceptibility of P. gingivalis to the different modes of photo-activated disinfection in planktonic culture. Methods: Cultures of P. gingivalis were assigned into three main groups of photothermal disinfection with 810 nm laser in continuous mode + EmunDo® (indocyanine green based photosensitizer), 808 nm laser in pulse mode + Perio green® (indocyanine green based photosensitizer); and antibacterial photodynamic therapy with 662 nm laser + Radachlorin® photosensitizer. Then, the bacterial viability was determined by serial dilution method by counting the number of colonies forming units. The results were analyzed by one-way analysis of variance and Tukey test (p < 0.05). Results: All three groups showed significant reduction in the viability of P. gingivalis (p < 0.001). However; this reduction was significantly higher in photothermal therapy with EmunDo®. Conclusions: Both methods of photodynamic and photothermal therapy were successful in Significant eradication of P. gingivalis. Although, photothermal approach was more efficient with EmunDo® in combination with 810 nm laser irradiation. © 2020 Elsevier B.V.
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