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The Effect of Indocyanine Green Antimicrobial Photothermal/Photodynamic Therapy on the Expression of Bcl-2 and Bax Messenger Rna Levels in Human Gingival Fibroblast Cells; [Влияние Антимикробной Фототермической / Фотодинамической Терапии Индоцианиновым Зелёным На Экспрессию Уровней Bcl-2 И Bax В Матричной Рнк В Клетках Фибробластов Десны Человек] Publisher Pubmed



Pourhajibagher M1 ; Gharesi S2 ; Chiniforush N3 ; Bahador A4
Authors
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Authors Affiliations
  1. 1. Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Genetics, Tehran Medical Branch, Islamic Azad University, Tehran, Iran
  3. 3. Laser Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Oral Microbiology Laboratory, Department of Medical Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Source: Folia Medica Published:2020


Abstract

Background: Antimicrobial photothermal/photodynamic therapy (PTT/PDT) with indocyanine green (ICG) is an adjuvant therapeutic approach in the treatment of periodontitis. To explore whether PTT/PDT with ICG causes cell death by apoptosis in human gingival fibroblast (HGF) cells, BAX and BCL-2 genes expression as key events for apoptosis were evaluated in this study. Materials and methods: HGF cells were treated with: 1) different concentrations (500-2000 μg/m L) of ICG alone, 2) Diode laser irradiation alone with a fluency of 39.06 J/cm2; 3) PTT/PDT combined different concentrations (500-2000 μg/mL ) of ICG with an 808 nm diode laser with a fluency of 39.06 J/cm2,and 4) controls (untreated cells). After that, BAX and BCL-2 messenger RNA levels were evaluated by real-time quantitative reverse transcription PCR. Results: PTT/PDT with 500 μg/mL of ICG caused significant increases in the expression of the BAX gene, with an 8.5-fold increase, which was approximately 7- and 8.5-fold higher than PTT/PDT with ICG for 1500 and 2000 μg/mL of ICG, respectively, indicating induction of apoptosis in HGF cells. ICG (in different test concentrations), diode laser, and PTT/PDT with ICG (1500 and 2000 μg/mL of ICG) treatment displayed insignificant increases in expression levels of BAX (all p >0.05). Our experiments showed an insignificant increase (1.1-1.6-fold) in the expression of BCL-2 after ICG, diode laser, and PTT/PDT with ICG treatment (all p >0.05). Conclusions: This study suggests that various concentration of ICG can be the diverse expression of BAX responses to PTT/PDT on HGF cells. © 2020 The Author(s).
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