99Mtc-Anti-Epidermal Growth Factor Receptor Nanobody for Tumor Imaging

99Mtc-Anti-Epidermal Growth Factor Receptor Nanobody for Tumor Imaging Publisher Pubmed

Piramoon M¹ ; Hosseinimehr SJ¹ ; Omidfar K^{2, 3} ; Noaparast Z¹ ; Abedi SM⁴

Show Affiliations

1. Department of Radiopharmacy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
2. Biosensor Research Center, Endocrinology and Metabolism Molecular Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
3. Endocrine and Metabolism Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran
4. Department of Radiology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

Source: Chemical Biology and Drug Design Published:2017

Abstract

Nanobodies are important biomolecules for tumor targeting. In this study, we synthesized and labeled anti-epidermal growth factor receptor (EGFR) nanobody OA-cb6 with 99mTc(CO)3 + and evaluated its characteristics for targeting the EGFR in the A431 human epidermal carcinoma cell line. Nanobody radiolabeling was achieved with high yield and radiochemical purity, and the radioconjugate was stable. Biodistribution results in nude mice exhibited a favorable tumor-to-muscle ratio at 4-hr postinjection, and tumor location was visualized at 4 hr after injection of radiolabeled nanobody. Our result showed that the OA-cb6-99mTc-tricarbonyl radiolabeled nanobody is a promising radiolabeled biomolecule for tumor imaging in cancers with high EGFR overexpression. © 2016 John Wiley & Sons A/S.

Style	Citing Format
MLA	Piramoon M, et al.. "99Mtc-Anti-Epidermal Growth Factor Receptor Nanobody for Tumor Imaging." Chemical Biology and Drug Design, vol. 89, no. 4, 2017, pp. 498-504.
APA	Piramoon M, Hosseinimehr SJ, Omidfar K, Noaparast Z, Abedi SM (2017). 99Mtc-Anti-Epidermal Growth Factor Receptor Nanobody for Tumor Imaging. Chemical Biology and Drug Design, 89(4), 498-504.
Chicago	Piramoon M, Hosseinimehr SJ, Omidfar K, Noaparast Z, Abedi SM. "99Mtc-Anti-Epidermal Growth Factor Receptor Nanobody for Tumor Imaging." Chemical Biology and Drug Design 89, no. 4 (2017): 498-504.
Harvard	Piramoon M et al. (2017) '99Mtc-Anti-Epidermal Growth Factor Receptor Nanobody for Tumor Imaging', Chemical Biology and Drug Design, 89(4), pp. 498-504.
Vancouver	Piramoon M, Hosseinimehr SJ, Omidfar K, Noaparast Z, Abedi SM. 99Mtc-Anti-Epidermal Growth Factor Receptor Nanobody for Tumor Imaging. Chemical Biology and Drug Design. 2017;89(4):498-504.
BibTex	@article{ author = {Piramoon M and Hosseinimehr SJ and Omidfar K and Noaparast Z and Abedi SM}, title = {99Mtc-Anti-Epidermal Growth Factor Receptor Nanobody for Tumor Imaging}, journal = {Chemical Biology and Drug Design}, volume = {89}, number = {4}, pages = {498-504}, year = {2017} }
RIS	TY - JOUR AU - Piramoon M AU - Hosseinimehr SJ AU - Omidfar K AU - Noaparast Z AU - Abedi SM TI - 99Mtc-Anti-Epidermal Growth Factor Receptor Nanobody for Tumor Imaging JO - Chemical Biology and Drug Design VL - 89 IS - 4 SP - 498 EP - 504 PY - 2017 ER -

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