Folate Conjugated Hyaluronic Acid Coated Alginate Nanogels Encapsulated Oxaliplatin Enhance Antitumor and Apoptosis Efficacy on Colorectal Cancer Cells (Ht29 Cell Line)

Folate Conjugated Hyaluronic Acid Coated Alginate Nanogels Encapsulated Oxaliplatin Enhance Antitumor and Apoptosis Efficacy on Colorectal Cancer Cells (Ht29 Cell Line) Publisher Pubmed

Shad PM¹ ; Karizi SZ² ; Javan RS² ; Mirzaie A³ ; Noorbazargan H⁴ ; Akbarzadeh I⁵ ; Rezaie H⁶

Show Affiliations

1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2. Department of Biology, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran
3. Department of Biology, Roudehen Branch, Islamic Azad University, Roudehen, Iran
4. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5. Department of Chemical and Petrochemical Engineering, Sharif University of Technology, Tehran, Iran
6. Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran

Source: Toxicology in Vitro Published:2020

Abstract

Oxaliplatin (OXA) has been widely used for treatment of colorectal cancer. In this study, to enhance antitumor and apoptosis efficacy, OXA was encapsulated in a novel folate conjugated hyaluronic acid coated alginate nanogels (F/HA/AL/OXA). The F/HA/AL/OXA nanogels were prepared by cross-linking process. The physico-chemical properties of F/HA/AL/OXA nanogels were characterized using scanning electron microscopy, transmission electron microscopy, fourier transform infrared spectroscopy, dynamic light scattering, and fluorescent spectrophotometry. The in-vitro antitumor activity of free OXA, AL, HA/AL, HA/AL/OXA and F/HA/AL/OXA nanogels were assessed using MTT assay against colorectal cancer cells (HT29 cell line). Finally, the effect of F/HA/AL/OXA nanogels on genes expression of Bax and Bcl2 was evaluated using quantitative real-time polymerase chain reaction (qRT-PCR) technique. The F/HA/AL/OXA nanogels were 200.3 nm in diameter and had a zeta potential of −22.0 mv. Antitumor activity of F/HA/AL/OXA nanogels on HT29 cell line indicated the highest antitumor activity as compared to free OXA and the empty nanogels. Compared to free OXA and the empty nanogels, the expression of Bax in HT29 cells treated with F/HA/AL/OXA nanogels was significantly increased along with suppression of Bcl-2 (p <. 01). In general, the present F/HA/AL/OXA nanogels are a promising carrier candidate for OXA to improve the anti-tumor activity. © 2019

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Style	Citing Format
MLA	Shad PM, et al.. "Folate Conjugated Hyaluronic Acid Coated Alginate Nanogels Encapsulated Oxaliplatin Enhance Antitumor and Apoptosis Efficacy on Colorectal Cancer Cells (Ht29 Cell Line)." Toxicology in Vitro, vol. 65, no. , 2020, pp. -.
APA	Shad PM, Karizi SZ, Javan RS, Mirzaie A, Noorbazargan H, Akbarzadeh I, Rezaie H (2020). Folate Conjugated Hyaluronic Acid Coated Alginate Nanogels Encapsulated Oxaliplatin Enhance Antitumor and Apoptosis Efficacy on Colorectal Cancer Cells (Ht29 Cell Line). Toxicology in Vitro, 65(), -.
Chicago	Shad PM, Karizi SZ, Javan RS, Mirzaie A, Noorbazargan H, Akbarzadeh I, Rezaie H. "Folate Conjugated Hyaluronic Acid Coated Alginate Nanogels Encapsulated Oxaliplatin Enhance Antitumor and Apoptosis Efficacy on Colorectal Cancer Cells (Ht29 Cell Line)." Toxicology in Vitro 65, no. (2020): -.
Harvard	Shad PM et al. (2020) 'Folate Conjugated Hyaluronic Acid Coated Alginate Nanogels Encapsulated Oxaliplatin Enhance Antitumor and Apoptosis Efficacy on Colorectal Cancer Cells (Ht29 Cell Line)', Toxicology in Vitro, 65(), pp. -.
Vancouver	Shad PM, Karizi SZ, Javan RS, Mirzaie A, Noorbazargan H, Akbarzadeh I, et al.. Folate Conjugated Hyaluronic Acid Coated Alginate Nanogels Encapsulated Oxaliplatin Enhance Antitumor and Apoptosis Efficacy on Colorectal Cancer Cells (Ht29 Cell Line). Toxicology in Vitro. 2020;65():-.
BibTex	@article{ author = {Shad PM and Karizi SZ and Javan RS and Mirzaie A and Noorbazargan H and Akbarzadeh I and Rezaie H}, title = {Folate Conjugated Hyaluronic Acid Coated Alginate Nanogels Encapsulated Oxaliplatin Enhance Antitumor and Apoptosis Efficacy on Colorectal Cancer Cells (Ht29 Cell Line)}, journal = {Toxicology in Vitro}, volume = {65}, number = {}, pages = {-}, year = {2020} }
RIS	TY - JOUR AU - Shad PM AU - Karizi SZ AU - Javan RS AU - Mirzaie A AU - Noorbazargan H AU - Akbarzadeh I AU - Rezaie H TI - Folate Conjugated Hyaluronic Acid Coated Alginate Nanogels Encapsulated Oxaliplatin Enhance Antitumor and Apoptosis Efficacy on Colorectal Cancer Cells (Ht29 Cell Line) JO - Toxicology in Vitro VL - 65 IS - SP - EP - PY - 2020 ER -

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