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Enhanced Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells by Diopside Coated Poly-L-Lactic Acid-Based Nanofibrous Scaffolds Publisher



Birhanu G1, 2, 3 ; Doostitelgerd M4 ; Zandikarimi A1 ; Karimi Z5 ; Porgham Daryasari M2, 6 ; Akbari Javar H2 ; Seyedjafari E1
Authors
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Authors Affiliations
  1. 1. Department of Biotechnology, University of Tehran, Tehran, Iran
  2. 2. Department of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Sciences, International campus (TUMS-IC), Tehran, Iran
  3. 3. School of Pharmacy, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia
  4. 4. Cell Therapy and Regenerative Medicine Comprehensive Center, Kerman University of Medical Sciences, Kerman, Iran
  5. 5. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  6. 6. Department of Pharmaceutical Biomaterials, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

Source: International Journal of Polymeric Materials and Polymeric Biomaterials Published:2022


Abstract

In this study, diopside coated Poly-L-lactic acid-based (PLLA/Diopside) and Poly-L-lactic acid along with pluronic acid (PLLA-P123/Diopside) scaffolds were fabricated by electrospinning to improve the drawbacks with pure polymer and pure bioceramic scaffolds in bone tissue engineering application. The surface morphology, hydrophilicity, and mechanical strength were evaluated. The cell proliferation, differentiation, and expression of osteo related genes were evaluated by measuring the basic osteogenic markers and real time polymerase chain reaction, respectively. Diopside coated Poly-L-lactic acid-based nanofibrous scaffolds have improved surface and biological properties making them a good promising candidate for proliferation and osteogenic differentiation in bone repair and regeneration compared to nanofibrous scaffolds without diopside. Especially, post plasma treated diopside coated PLLA demonstrated better osteogenesis and expression of osteo related genes than the other groups. Although, diopside coated PLLA-P123 composite in comparison with PLLA-Plasma/diopside scaffolds were showed less osteogenesis and expression of osteo related genes, but the results were comparable. Therefore, diopside coated (PLLA-plasma and PLLA/P123 composite) scaffolds could be ideal cost effective grafts in bone repair and replacement therapy. © 2021 Taylor & Francis Group, LLC.
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