Development of Anti-Cd47 Single-Chain Variable Fragment Targeted Magnetic Nanoparticles for Treatment of Human Bladder Cancer

Development of Anti-Cd47 Single-Chain Variable Fragment Targeted Magnetic Nanoparticles for Treatment of Human Bladder Cancer Publisher Pubmed

Rezaei G¹ ; Habibianbouhi M¹ ; Mahmoudi M² ; Azadmanesh K³ ; Moradikalbolandi S¹ ; Behdani M⁴ ; Ghazizadeh L¹ ; Abolhassani M⁵ ; Shokrgozar MA¹

Show Affiliations

1. National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran
2. Nanotechnology Research Center and Department of Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, 14155-6451, Iran
3. Virology Deparment, Pasteur Institute of Iran, Tehran, Iran
4. Biotechnology Research Center, Venom and Biotherapeutics Molecules Lab, Pasteur Institute of Iran, Tehran, Iran
5. Immunology Department, Hybridoma Lab, Pasteur Institute of Iran, Tehran, Iran

Source: Nanomedicine Published:2017

Abstract

Aim: To develop a novel anti-CD47 single-chain variable fragment (scFv) functionalized magnetic nanoparticles (MNPs) for targeting bladder cell lines and its applicability in thermotherapy. Material & methods: An immunized murine antibody phage display library was constructed and screened to isolate anti-CD47 binders. A scFv was selected and conjugated to MNPs which was then utilized to discriminate CD47+ bladder cells along with assessing its efficacy in thermotherapy. Results: An scFv with high affinity to bladder cells was efficiently conjugated to MNPs. Following a hyperthermia treatment, the function of scFv-MNP conjugates led to a considerable reduction in cell viability. Conclusion: The anti-CD47 scFv-MNP conjugate was an effective cancer cell thermotherapy tool that might pave the way for development of bionano-based targeting techniques in both early detection and treatment of cancer. © 2017 Future Medicine Ltd.

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Style	Citing Format
MLA	Rezaei G, et al.. "Development of Anti-Cd47 Single-Chain Variable Fragment Targeted Magnetic Nanoparticles for Treatment of Human Bladder Cancer." Nanomedicine, vol. 12, no. 6, 2017, pp. 597-613.
APA	Rezaei G, Habibianbouhi M, Mahmoudi M, Azadmanesh K, Moradikalbolandi S, Behdani M, Ghazizadeh L, Abolhassani M, Shokrgozar MA (2017). Development of Anti-Cd47 Single-Chain Variable Fragment Targeted Magnetic Nanoparticles for Treatment of Human Bladder Cancer. Nanomedicine, 12(6), 597-613.
Chicago	Rezaei G, Habibianbouhi M, Mahmoudi M, Azadmanesh K, Moradikalbolandi S, Behdani M, Ghazizadeh L, Abolhassani M, Shokrgozar MA. "Development of Anti-Cd47 Single-Chain Variable Fragment Targeted Magnetic Nanoparticles for Treatment of Human Bladder Cancer." Nanomedicine 12, no. 6 (2017): 597-613.
Harvard	Rezaei G et al. (2017) 'Development of Anti-Cd47 Single-Chain Variable Fragment Targeted Magnetic Nanoparticles for Treatment of Human Bladder Cancer', Nanomedicine, 12(6), pp. 597-613.
Vancouver	Rezaei G, Habibianbouhi M, Mahmoudi M, Azadmanesh K, Moradikalbolandi S, Behdani M, et al.. Development of Anti-Cd47 Single-Chain Variable Fragment Targeted Magnetic Nanoparticles for Treatment of Human Bladder Cancer. Nanomedicine. 2017;12(6):597-613.
BibTex	@article{ author = {Rezaei G and Habibianbouhi M and Mahmoudi M and Azadmanesh K and Moradikalbolandi S and Behdani M and Ghazizadeh L and Abolhassani M and Shokrgozar MA}, title = {Development of Anti-Cd47 Single-Chain Variable Fragment Targeted Magnetic Nanoparticles for Treatment of Human Bladder Cancer}, journal = {Nanomedicine}, volume = {12}, number = {6}, pages = {597-613}, year = {2017} }
RIS	TY - JOUR AU - Rezaei G AU - Habibianbouhi M AU - Mahmoudi M AU - Azadmanesh K AU - Moradikalbolandi S AU - Behdani M AU - Ghazizadeh L AU - Abolhassani M AU - Shokrgozar MA TI - Development of Anti-Cd47 Single-Chain Variable Fragment Targeted Magnetic Nanoparticles for Treatment of Human Bladder Cancer JO - Nanomedicine VL - 12 IS - 6 SP - 597 EP - 613 PY - 2017 ER -

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