Mir-199A-3P Modulates Mtor and Pak4 Pathways and Inhibits Tumor Growth in a Hepatocellular Carcinoma Transgenic Mouse Model

Mir-199A-3P Modulates Mtor and Pak4 Pathways and Inhibits Tumor Growth in a Hepatocellular Carcinoma Transgenic Mouse Model Publisher

Callegari E¹ ; Dabundo L¹ ; Guerriero P¹ ; Simioni C¹ ; Elamin BK^{2, 3} ; Russo M¹ ; Cani A¹ ; Bassi C¹ ; Zagatti B¹ ; Giacomelli L⁴ ; Blandamura S⁴ ; Moshiri F^{1, 5} ; Ultimo S¹ ; Frassoldati A¹ Show All Authors

Callegari E¹
Dabundo L¹
Guerriero P¹
Simioni C¹
Elamin BK^{2, 3}
Russo M¹
Cani A¹
Bassi C¹
Zagatti B¹
Giacomelli L⁴
Blandamura S⁴
Moshiri F^{1, 5}
Ultimo S¹
Frassoldati A¹
Altavilla G⁴
Gramantieri L⁶
Neri LM¹
Sabbioni S⁷
Negrini M¹

Show Affiliations

1. Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, 44121, Italy
2. Department of Basic Sciences, College of Medicine, University of Bisha, Bisha, 61922, Saudi Arabia
3. Microbiology Department, Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, 11115, Sudan
4. Department of Medicine DIMED, University of Padova, Padova, 35128, Italy
5. Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, 11369, Iran
6. Center for Applied Biomedical Research, St. Orsola-Malpighi University Hospital, Bologna, 40138, Italy
7. Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, 44121, Italy

Source: Molecular Therapy Nucleic Acids Published:2018

Abstract

Hepatocellular carcinoma (HCC) is the second leading cause of cancer-related death worldwide. Prognosis is poor, and therapeutic options are limited. MicroRNAs (miRNAs) have emerged as potential therapeutic molecules against cancer. Here, we investigated the therapeutic efficacy of miR-199a-3p, an miRNA highly expressed in normal liver and downregulated in virtually all HCCs. The therapeutic value of miR-199a-3p mimic molecules was assayed in the TG221 mouse, a transgenic model highly predisposed to the development of liver cancer. Administration of miR-199a-3p mimics in the TG221 transgenic mouse showing liver cancer led to a significant reduction of number and size of tumor nodules compared to control animals. In vivo delivery confirmed protein downregulation of the miR-199a-3p direct targets, mechanistic target of rapamycin (MTOR) and p21 activated kinase 4 (PAK4), ultimately leading to the repression of FOXM1. Remarkably, the anti-tumor activity of miR-199a-3p mimics was comparable to that obtained with sorafenib. These results suggested that miR-199a-3p may be considered a promising HCC therapeutic option. © 2018 The Author(s)

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Style	Citing Format
MLA	Callegari E, et al.. "Mir-199A-3P Modulates Mtor and Pak4 Pathways and Inhibits Tumor Growth in a Hepatocellular Carcinoma Transgenic Mouse Model." Molecular Therapy Nucleic Acids, vol. 11, no. , 2018, pp. 485-493.
APA	Callegari E, Dabundo L, Guerriero P, Simioni C, Elamin BK, Russo M, Cani A, Bassi C, Zagatti B, Giacomelli L, Blandamura S, Moshiri F, Ultimo S, Frassoldati A, Altavilla G, Gramantieri L, Neri LM, Sabbioni S, Negrini M (2018). Mir-199A-3P Modulates Mtor and Pak4 Pathways and Inhibits Tumor Growth in a Hepatocellular Carcinoma Transgenic Mouse Model. Molecular Therapy Nucleic Acids, 11(), 485-493.
Chicago	Callegari E, Dabundo L, Guerriero P, Simioni C, Elamin BK, Russo M, Cani A, et al.. "Mir-199A-3P Modulates Mtor and Pak4 Pathways and Inhibits Tumor Growth in a Hepatocellular Carcinoma Transgenic Mouse Model." Molecular Therapy Nucleic Acids 11, no. (2018): 485-493.
Harvard	Callegari E et al. (2018) 'Mir-199A-3P Modulates Mtor and Pak4 Pathways and Inhibits Tumor Growth in a Hepatocellular Carcinoma Transgenic Mouse Model', Molecular Therapy Nucleic Acids, 11(), pp. 485-493.
Vancouver	Callegari E, Dabundo L, Guerriero P, Simioni C, Elamin BK, Russo M, et al.. Mir-199A-3P Modulates Mtor and Pak4 Pathways and Inhibits Tumor Growth in a Hepatocellular Carcinoma Transgenic Mouse Model. Molecular Therapy Nucleic Acids. 2018;11():485-493.
BibTex	@article{ author = {Callegari E and Dabundo L and Guerriero P and Simioni C and Elamin BK and Russo M and Cani A and Bassi C and Zagatti B and Giacomelli L and Blandamura S and Moshiri F and Ultimo S and Frassoldati A and Altavilla G and Gramantieri L and Neri LM and Sabbioni S and Negrini M}, title = {Mir-199A-3P Modulates Mtor and Pak4 Pathways and Inhibits Tumor Growth in a Hepatocellular Carcinoma Transgenic Mouse Model}, journal = {Molecular Therapy Nucleic Acids}, volume = {11}, number = {}, pages = {485-493}, year = {2018} }
RIS	TY - JOUR AU - Callegari E AU - Dabundo L AU - Guerriero P AU - Simioni C AU - Elamin BK AU - Russo M AU - Cani A AU - Bassi C AU - Zagatti B AU - Giacomelli L AU - Blandamura S AU - Moshiri F AU - Ultimo S AU - Frassoldati A AU - Altavilla G AU - Gramantieri L AU - Neri LM AU - Sabbioni S AU - Negrini M TI - Mir-199A-3P Modulates Mtor and Pak4 Pathways and Inhibits Tumor Growth in a Hepatocellular Carcinoma Transgenic Mouse Model JO - Molecular Therapy Nucleic Acids VL - 11 IS - SP - 485 EP - 493 PY - 2018 ER -

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