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Effects of Antioxidants, Catalase and Α-Tocopherol on Cell Viability and Oxidative Stress Variables in Frozen-Thawed Mice Spermatogonial Stem Cells Publisher Pubmed



Aliakbari F1 ; Sedighi Gilani MA2, 3 ; Yazdekhasti H1 ; Koruji M6 ; Asgari HR7 ; Baazm M4 ; Izadyar F5 ; Kharrazi Nejad E1 ; Khanezad M1 ; Abbasi M1
Authors
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Authors Affiliations
  1. 1. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Urology, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Andrology, Reproductive Biomedicine Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
  4. 4. Department of Anatomy, School of Medicine, Arak University of Medical Sciences, Arak, Iran
  5. 5. PrimeGen Biotech LLC, Santa Ana, CA, United States
  6. 6. Cellular and Molecular Research Center & Department of Anatomical Sciences, Iran University of Medical Sciences, Tehran, Iran
  7. 7. Department of Anatomical Sciences, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran

Source: Artificial Cells# Nanomedicine and Biotechnology Published:2017


Abstract

Cryopreservation of spermatogonial stem cells is considered as a useful procedure for preserving fertility in children with testis cancer. SSCs were isolated from testes mice, and then antioxidant was added to the freezing medium. The Bax expression level in antioxidant groups was significantly (P ≤ 0.05) lower than the control group and a significant rise of Bcl2 expression was detected in the antioxidant groups. ROS production with antioxidant was significantly lower compared with the control group. Cryopreservation with the addition of the antioxidants can help increase the number of SSCs and improve the quality and viability of these cells after cryopreservation. © 2016 Informa UK Limited, trading as Taylor & Francis Group.
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