Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Antioxidant Effects of Quercetin in Freeze-Thawing Process of Mouse Spermatogonial Stem Cells Publisher



Amidi F1 ; Rashidi Z1 ; Khosravizadeh Z1 ; Khodamoradi K1 ; Talebi A1 ; Navid S1 ; Abbasi M1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Poursina St, Keshavarse Ave, Tehran, Iran

Source: Asian Pacific Journal of Reproduction Published:2019


Abstract

Objective: To evaluate the antioxidant effect of quercetin on cell viability, reactive oxygen species (ROS) contents and apoptosis of cryopreserved mouse spermatogonial stem cells (mSSCs). Methods: mSSCs were isolated from neonate mice and cultivated in culture medium containing 30 μ quercetin for 48 h and then frozen for 2 weeks. After thawing, MTT assay was carried out to analyze the cell viability. Moreover, intracellular ROS levels were measured by flow cytometery and apoptosis was evaluated by detection of phosphatidylserine externalization assay and also real-time polymerase chain reaction. Results: Pre-treatment of mSSCs by 30 μ quercetin significantly decreased intracellular ROS content and apoptotic cell numbers and improved viability of mSSCs. Moreover, the gene expression of Bcl-2 and Bax significantly increased and decreased respectively after the freeze-thawing process. Conclusions: Pre-treatment of mSSCs with quercetin can improve cell viability and reduce apoptosis during freeze-thawing process. It can be a promising way to improve the quality and efficiency of cryopreservation protocols used in fertility preservation strategies. © 2019 Asian Pacific Journal of Reproduction Produced by Wolters Kluwer-Medknow.
Related Docs
View other Related Docs
1. Recovering Spermatogenesis by Protected Cryopreservation Using Metformin and Transplanting Spermatogonial Stem Cells Into Testis in an Azoospermia Mouse Model, Biopreservation and Biobanking (2024)
2. Effect of Mir-30A-5P on Apoptosis, Colonization, and Oxidative Stress Variables in Frozen-Thawed Neonatal Mice Spermatogonial Stem Cells, Biopreservation and Biobanking (2021)
3. Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of Pcl/Gel Nanofibers; [Proliferacion Y Diferenciacion De Celulas Madre Espermatogonicas De Raton En Una Superficie Tridimensional Compuesta De Nanofibras Pcl/Gel], International Journal of Morphology (2019)
4. The Role of Antioxidants in Sperm Freezing: A Review, Cell and Tissue Banking (2016)
5. Effects of Antioxidants, Catalase and Α-Tocopherol on Cell Viability and Oxidative Stress Variables in Frozen-Thawed Mice Spermatogonial Stem Cells, Artificial Cells# Nanomedicine and Biotechnology (2017)
6. Effect of a Freezing Medium Containing Melatonin on Markers of Pre-Meiotic and Post-Meiotic Spermatogonial Stem Cells (Sscs) After Transplantation in an Azoospermia Mouse Model Due to Testicular Torsion, Reproductive Sciences (2021)
7. Melatonin in Cryopreservation Media Improves Transplantation Efficiency of Frozen–Thawed Spermatogonial Stem Cells Into Testes of Azoospermic Mice, Stem Cell Research and Therapy (2022)
8. Improving the Efficacy of Cryopreservation of Spermatogonia Stem Cells by Antioxidant Supplements, Cellular Reprogramming (2016)
Experts (# of related papers)
View all Related Experts
Mehdi Abbasi (6)
Fardin Amidi (4)
Other Related Docs
9. Improved Isolation, Proliferation, and Differentiation Capacity of Mouse Ovarian Putative Stem Cells, Cellular Reprogramming (2017)
10. The Effects of Melatonin on Colonization of Neonate Spermatogonial Mouse Stem Cells in a Three-Dimensional Soft Agar Culture System, Stem Cell Research and Therapy (2017)
11. Colonization of Neonate Mouse Spermatogonial Stem Cells Co-Culture With Sertoli Cells in the Presence and Absence Soft Agar, Asian Pacific Journal of Reproduction (2020)
12. Application of Tissue-Specific Extracellular Matrix in Tissue Engineering: Focus on Male Fertility Preservation, Reproductive Sciences (2022)
13. Effect of Sulforaphane on Apoptosis, Reactive Oxygen Species and Lipids Peroxidation of Human Sperm During Cryopreservation, Cryobiology (2021)
14. Coenzyme Q10 Supplementation and Oxidative Stress Parameters: A Systematic Review and Meta-Analysis of Clinical Trials, European Journal of Clinical Pharmacology (2020)
15. Antioxidant Effect of Melatonin on Proliferation, Apoptosis, and Oxidative Stress Variables in Frozen-Thawed Neonatal Mice Spermatogonial Stem Cells, Biopreservation and Biobanking (2022)
16. Germline Cells Derived From Mesenchymal Stem Cells, With the Focus on Wharton's Jelly, Asian Pacific Journal of Reproduction (2018)
17. Astaxanthin Relieves Busulfan-Induced Oxidative Apoptosis in Cultured Human Spermatogonial Stem Cells by Activating the Nrf-2/Ho-1 Pathway, Reproductive Sciences (2022)
18. Female Fertility Preservation, Clinical and Experimental Options, Journal of Reproduction and Infertility (2018)