Bi-Functionalized Aminoguanidine-Pegylated Periodic Mesoporous Organosilica Nanoparticles: A Promising Nanocarrier for Delivery of Cas9-Sgrna Ribonucleoproteine Publisher Pubmed



Salekdeh PR¹ ; Mamani L² ; Tavakkolybazzaz J¹ ; Mousavi H¹ ; Modarressi MH¹ ; Salekdeh GH^{3, 4} Show Affiliations
Source: Journal of Nanobiotechnology Published:2021

Abstract

Background: There is a great interest in the efficient intracellular delivery of Cas9-sgRNA ribonucleoprotein complex (RNP) and its possible applications for in vivo CRISPR-based gene editing. In this study, a nanoporous mediated gene-editing approach has been successfully performed using a bi-functionalized aminoguanidine-PEGylated periodic mesoporous organosilica (PMO) nanoparticles (RNP@AGu@PEG1500-PMO) as a potent and biocompatible nanocarrier for RNP delivery. Results: The bi-functionalized MSN-based nanomaterials have been fully characterized using electron microscopy (TEM and SEM), nitrogen adsorption measurements, thermogravimetric analysis (TGA), X-ray powder diffraction (XRD), Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR), and dynamic light scattering (DLS). The results confirm that AGu@PEG1500-PMO can be applied for gene-editing with an efficiency of about 40% as measured by GFP gene knockdown of HT1080-GFP cells with no notable change in the morphology of the cells. Conclusions: Due to the high stability and biocompatibility, simple synthesis, and cost-effectiveness, the developed bi-functionalized PMO-based nano-network introduces a tailored nanocarrier that has remarkable potential as a promising trajectory for biomedical and RNP delivery applications. [Figure not available: see fulltext.] © 2021, The Author(s).