Synthesis and Characterization of Vitamin D3-Functionalized Carbon Dots for Crispr/Cas9 Delivery

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Synthesis and Characterization of Vitamin D3-Functionalized Carbon Dots for Crispr/Cas9 Delivery Publisher Pubmed

Hasanzadeh A^{1, 2, 3} ; Radmanesh F^{4, 5} ; Hosseini ES^{1, 2} ; Hashemzadeh I^{1, 2, 3} ; Kiani J^{6, 7} ; Naseri M^{6, 7} ; Nourizadeh H³ ; Fatahi Y^{8, 9, 10} ; Azar BKY^{7, 11} ; Marani BG^{1, 2, 3} ; Beyzavi A¹² ; Mahabadi VP^{1, 13} ; Karimi M^{1, 2, 3, 6, 14, 15}

Show Affiliations

1. Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, 1449614535, Iran
2. Department of Medical Nanotechnology, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, 1449614535, Iran
3. Advanced Nanobiotechnology and Nanomedicine Research Group (ANNRG), Iran University of Medical Sciences, Tehran, 1449614535, Iran
4. Uro-Oncology Research Center, Tehran University of Medical Sciences, Tehran, 1449614535, Iran
5. Department of Cell Engineering, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, Acecr, Tehran, 1665659911, Iran
6. Oncopathology Research Center, Iran University of Medical Sciences, Tehran, 1449614535, Iran
7. Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, 1449614535, Iran
8. Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, 1417613151, Iran
9. Department of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, 1417613151, Iran
10. Universal Scientific Education and Research Network (USERN), Tehran, 1417755331, Iran
11. Research Committee, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, 1449614535, Iran
12. Department of Mechanical Engineering, Boston University, Boston, 02215, MA, United States
13. Neuroscience Research Center, Iran University of Medical Sciences, Tehran, 1449614535, Iran
14. Research Center for Science and Technology in Medicine, Tehran University of Medical Sciences, Tehran, 1417613151, Iran
15. Applied Biotechnology Research Centre, Tehran Medical Science, Islamic Azad University, Tehran, 1916893813, Iran

Source: Nanomedicine Published:2021

Abstract

Aim: To develop a novel nanovector for the delivery of genetic fragments and CRISPR/Cas9 systems in particular. Materials and methods: Vitamin D3-functionalized carbon dots (D/CDs) fabricated using one-step microwave-Aided methods were characterized by different microscopic and spectroscopic techniques. The 3-(4,5-dimethyl-2-Thiazolyl)-2,5-diphenyl tetrazolium bromide assay and flow cytometry were employed to determine the cell viability and transfection efficiency. Results: D/CDs transfected CRISPR plasmid in various cell lines with high efficiency while maintaining their remarkable efficacy at high serum concentration and low plasmid doses. They also showed great potential for the green fluorescent protein disruption by delivering two different types of CRISPR/Cas9 systems. Conclusion: Given their high efficiency and safety, D/CDs provide a versatile gene-delivery vector for clinical applications. © 2021 Future Medicine Ltd.. All rights reserved.

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Style	Citing Format
MLA	Hasanzadeh A, et al.. "Synthesis and Characterization of Vitamin D3-Functionalized Carbon Dots for Crispr/Cas9 Delivery." Nanomedicine, vol. 16, no. 19, 2021, pp. 1673-1690.
APA	Hasanzadeh A, Radmanesh F, Hosseini ES, Hashemzadeh I, Kiani J, Naseri M, Nourizadeh H, Fatahi Y, Azar BKY, Marani BG, Beyzavi A, Mahabadi VP, Karimi M (2021). Synthesis and Characterization of Vitamin D3-Functionalized Carbon Dots for Crispr/Cas9 Delivery. Nanomedicine, 16(19), 1673-1690.
Chicago	Hasanzadeh A, Radmanesh F, Hosseini ES, Hashemzadeh I, Kiani J, Naseri M, Nourizadeh H, et al.. "Synthesis and Characterization of Vitamin D3-Functionalized Carbon Dots for Crispr/Cas9 Delivery." Nanomedicine 16, no. 19 (2021): 1673-1690.
Harvard	Hasanzadeh A et al. (2021) 'Synthesis and Characterization of Vitamin D3-Functionalized Carbon Dots for Crispr/Cas9 Delivery', Nanomedicine, 16(19), pp. 1673-1690.
Vancouver	Hasanzadeh A, Radmanesh F, Hosseini ES, Hashemzadeh I, Kiani J, Naseri M, et al.. Synthesis and Characterization of Vitamin D3-Functionalized Carbon Dots for Crispr/Cas9 Delivery. Nanomedicine. 2021;16(19):1673-1690.
BibTex	@article{ author = {Hasanzadeh A and Radmanesh F and Hosseini ES and Hashemzadeh I and Kiani J and Naseri M and Nourizadeh H and Fatahi Y and Azar BKY and Marani BG and Beyzavi A and Mahabadi VP and Karimi M}, title = {Synthesis and Characterization of Vitamin D3-Functionalized Carbon Dots for Crispr/Cas9 Delivery}, journal = {Nanomedicine}, volume = {16}, number = {19}, pages = {1673-1690}, year = {2021} }
RIS	TY - JOUR AU - Hasanzadeh A AU - Radmanesh F AU - Hosseini ES AU - Hashemzadeh I AU - Kiani J AU - Naseri M AU - Nourizadeh H AU - Fatahi Y AU - Azar BKY AU - Marani BG AU - Beyzavi A AU - Mahabadi VP AU - Karimi M TI - Synthesis and Characterization of Vitamin D3-Functionalized Carbon Dots for Crispr/Cas9 Delivery JO - Nanomedicine VL - 16 IS - 19 SP - 1673 EP - 1690 PY - 2021 ER -

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