Synthesis and Characterization of Vitamin D3-Functionalized Carbon Dots for Crispr/Cas9 Delivery Publisher Pubmed



Hasanzadeh A^{1, 2, 3} ; Radmanesh F^{4, 5} ; Hosseini ES^{1, 2} ; Hashemzadeh I^{1, 2, 3} ; Kiani J^{6, 7} ; Naseri M^{6, 7} ; Nourizadeh H³ ; Fatahi Y^{8, 9, 10} ; Azar BKY^{7, 11} ; Marani BG^{1, 2, 3} ; Beyzavi A¹² ; Mahabadi VP^{1, 13} ; Karimi M^{1, 2, 3, 6, 14, 15}
Source: Nanomedicine Published:2021

Abstract

Aim: To develop a novel nanovector for the delivery of genetic fragments and CRISPR/Cas9 systems in particular. Materials and methods: Vitamin D3-functionalized carbon dots (D/CDs) fabricated using one-step microwave-Aided methods were characterized by different microscopic and spectroscopic techniques. The 3-(4,5-dimethyl-2-Thiazolyl)-2,5-diphenyl tetrazolium bromide assay and flow cytometry were employed to determine the cell viability and transfection efficiency. Results: D/CDs transfected CRISPR plasmid in various cell lines with high efficiency while maintaining their remarkable efficacy at high serum concentration and low plasmid doses. They also showed great potential for the green fluorescent protein disruption by delivering two different types of CRISPR/Cas9 systems. Conclusion: Given their high efficiency and safety, D/CDs provide a versatile gene-delivery vector for clinical applications. © 2021 Future Medicine Ltd.. All rights reserved.