Prospective Isolation of Isl1+ Cardiac Progenitors From Human Escs for Myocardial Infarction Therapy

Prospective Isolation of Isl1+ Cardiac Progenitors From Human Escs for Myocardial Infarction Therapy Publisher Pubmed

Ghazizadeh Z^{1, 2} ; Fattahi F^{3, 13} ; Mirzaei M^{4, 5, 6} ; Bayersaikhan D⁷ ; Lee J⁷ ; Chae S⁸ ; Hwang D⁸ ; Byun K⁷ ; Tabar MS³ ; Taleahmad S³ ; Mirshahvaladi S³ ; Shabani P¹ ; Fonoudi H¹ ; Haynes PA⁴ Show All Authors

Ghazizadeh Z^{1, 2}
Fattahi F^{3, 13}
Mirzaei M^{4, 5, 6}
Bayersaikhan D⁷
Lee J⁷
Chae S⁸
Hwang D⁸
Byun K⁷
Tabar MS³
Taleahmad S³
Mirshahvaladi S³
Shabani P¹
Fonoudi H¹
Haynes PA⁴
Baharvand H^{1, 9}
Aghdami N^{1, 10}
Evans T¹¹
Lee B⁷
Salekdeh GH^{3, 12}

Show Affiliations

1. Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
2. School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
3. Department of Molecular Systems Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Banihashem Square, Banihashem Street, Ressalat Highway, Tehran, Iran
4. Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, NSW, Australia
5. Faculty of Medicine and Health Sciences, Macquarie University, Sydney, NSW, Australia
6. Australian Proteome Analysis Facility, Macquarie University, Sydney, NSW, Australia
7. Center for Regenerative Medicine, Gachon University, Incheon City, South Korea
8. Department of New Biology and Center for Plant Aging Research, Institute for Basic Science, Daegu Gyeongbuk Institute of Science and Technology, Daegu, South Korea
9. Department of Developmental Biology, University of Science and Culture, ACECR, Tehran, Iran
10. Department of Regenerative Biomedicine at Cell Science Research, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
11. Department of Surgery, Weill Cornell Medicine, New York, NY, United States
12. Department of Molecular Sciences, Macquarie University, Sydney, NSW, Australia
13. Department of Biochemistry and Biophysics, Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, School of Medicine, University of California, San Francisco, United States

Source: Stem Cell Reports Published:2018

Abstract

The LIM-homeodomain transcription factor ISL1 marks multipotent cardiac progenitors that give rise to cardiac muscle, endothelium, and smooth muscle cells. ISL1+ progenitors can be derived from human pluripotent stem cells, but the inability to efficiently isolate pure populations has limited their characterization. Using a genetic selection strategy, we were able to highly enrich ISL1+ cells derived from human embryonic stem cells. Comparative quantitative proteomic analysis of enriched ISL1+ cells identified ALCAM (CD166) as a surface marker that enabled the isolation of ISL1+ progenitor cells. ALCAM+/ISL1+ progenitors are multipotent and differentiate into cardiomyocytes, endothelial cells, and smooth muscle cells. Transplantation of ALCAM+ progenitors enhances tissue recovery, restores cardiac function, and improves angiogenesis through activation of AKT-MAPK signaling in a rat model of myocardial infarction, based on cardiac MRI and histology. Our study establishes an efficient method for scalable purification of human ISL1+ cardiac precursor cells for therapeutic applications. In this article, Salekdeh and colleagues show that ISL1+ cardiac progenitors can be purified from a heterogeneous population of hESC-derived cardiomyocytes using ALCAM. Transplantation of multipotent ISL1+/ALCAM+ progenitors enhances tissue recovery, restores cardiac function, and improves angiogenesis in a rat model of myocardial infarction, based on cardiac MRI and histology. © 2018 The Authors

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Style	Citing Format
MLA	Ghazizadeh Z, et al.. "Prospective Isolation of Isl1+ Cardiac Progenitors From Human Escs for Myocardial Infarction Therapy." Stem Cell Reports, vol. 10, no. 3, 2018, pp. 848-859.
APA	Ghazizadeh Z, Fattahi F, Mirzaei M, Bayersaikhan D, Lee J, Chae S, Hwang D, Byun K, Tabar MS, Taleahmad S, Mirshahvaladi S, Shabani P, Fonoudi H, Haynes PA, Baharvand H, Aghdami N, Evans T, Lee B, Salekdeh GH (2018). Prospective Isolation of Isl1+ Cardiac Progenitors From Human Escs for Myocardial Infarction Therapy. Stem Cell Reports, 10(3), 848-859.
Chicago	Ghazizadeh Z, Fattahi F, Mirzaei M, Bayersaikhan D, Lee J, Chae S, Hwang D, et al.. "Prospective Isolation of Isl1+ Cardiac Progenitors From Human Escs for Myocardial Infarction Therapy." Stem Cell Reports 10, no. 3 (2018): 848-859.
Harvard	Ghazizadeh Z et al. (2018) 'Prospective Isolation of Isl1+ Cardiac Progenitors From Human Escs for Myocardial Infarction Therapy', Stem Cell Reports, 10(3), pp. 848-859.
Vancouver	Ghazizadeh Z, Fattahi F, Mirzaei M, Bayersaikhan D, Lee J, Chae S, et al.. Prospective Isolation of Isl1+ Cardiac Progenitors From Human Escs for Myocardial Infarction Therapy. Stem Cell Reports. 2018;10(3):848-859.
BibTex	@article{ author = {Ghazizadeh Z and Fattahi F and Mirzaei M and Bayersaikhan D and Lee J and Chae S and Hwang D and Byun K and Tabar MS and Taleahmad S and Mirshahvaladi S and Shabani P and Fonoudi H and Haynes PA and Baharvand H and Aghdami N and Evans T and Lee B and Salekdeh GH}, title = {Prospective Isolation of Isl1+ Cardiac Progenitors From Human Escs for Myocardial Infarction Therapy}, journal = {Stem Cell Reports}, volume = {10}, number = {3}, pages = {848-859}, year = {2018} }
RIS	TY - JOUR AU - Ghazizadeh Z AU - Fattahi F AU - Mirzaei M AU - Bayersaikhan D AU - Lee J AU - Chae S AU - Hwang D AU - Byun K AU - Tabar MS AU - Taleahmad S AU - Mirshahvaladi S AU - Shabani P AU - Fonoudi H AU - Haynes PA AU - Baharvand H AU - Aghdami N AU - Evans T AU - Lee B AU - Salekdeh GH TI - Prospective Isolation of Isl1+ Cardiac Progenitors From Human Escs for Myocardial Infarction Therapy JO - Stem Cell Reports VL - 10 IS - 3 SP - 848 EP - 859 PY - 2018 ER -

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