Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Identification of Catecholamine Neurotransmitters Using Fluorescence Sensor Array Publisher Pubmed



Ghasemi F1 ; Hormozinezhad MR1, 2 ; Mahmoudi M3, 4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Chemistry, Sharif University of Technology, Tehran, 11155-9516, Iran
  2. 2. Institute for Nanoscience and Nanotechnology, Sharif University of Technology, Tehran, Iran
  3. 3. Department of Nanotechnology and Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehra, 13169-43551, Iran
  4. 4. Division of Cardiovascular Medicine, Stanford University School of Medicine, Stanford, 94305-5101, CA, United States

Source: Analytica Chimica Acta Published:2016


Abstract

A nano-based sensor array has been developed for identification and discrimination of catecholamine neurotransmitters based on optical properties of their oxidation products under alkaline conditions. To produce distinct fluorescence response patterns for individual catecholamine, quenching of thioglycolic acid functionalized cadmium telluride (CdTe) quantum dots, by oxidation products, were employed along with the variation of fluorescence spectra of oxidation products. The spectral changes were analyzed with hierarchical cluster analysis (HCA) and principal component analysis (PCA) to identify catecholamine patterns. The proposed sensor could efficiently discriminate the individual catecholamine (i.e., dopamine, norepinephrine, and l-DOPA) and their mixtures in the concentration range of 0.25-30 μmol L-1. Finally, we found that the sensor had capability to identify the various catecholamines in urine sample. © 2016 Elsevier B.V.