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Mesenchymal Stem Cell-Derived Exosomes As Natural Nanoparticles (Nps) Stimulate the Growth of Limbal Stem Cells (Lscs) by Regulating Wnt/Β-Catenin, P38 Mpak and Erk Pathway Publisher



Samiei A1 ; Aliabadi P2 ; Parandavar M3 ; Shirvani M4 ; Firozabdi MSH5 ; Fazlinejad N6
Authors
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Authors Affiliations
  1. 1. Oral and Maxillofacial Pathology Department, Shiraz University of Dental Science, Fars, Shiraz, Iran
  2. 2. Department of Immunology and Biology, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Acute Surgical Department, Rajaee Hospital, Shiraz University of Medical Science, Shiraz, Iran
  4. 4. Poostchi Ophthalmology Research Center, Department of Ophthalmology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
  5. 5. Department of Optometry, School of Rehabilitation Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  6. 6. Department of Ophthalmology, Khodadoust Eye Hospital, Shiraz, Iran

Source: Nanomedicine Research Journal Published:2022


Abstract

Objective(s): Inthecurrent study, we investigated the impacts of the mesenchymal stem cell (MSCs)-exosome on limbal stem cells (LSCs) proliferation. Methods: Exosomes firstly were isolated from the human MSCs. Then, they characterized by expression of CD9, CD63 and CD81using the western blotting and morphological evaluation by transmission electron microscopy (TEM) image. The proliferation levels of treated LSCs were investigated following treatment with MSCs-exosome by MTT assay during the 1-4 days of exposure at 50-400 ng/ml concentrations. The expression levels of the beta-catenin, Wnt, p38 MAPK, and ERK were measured in LSCs within 12 and 96 hours of exposure. Results: MTT assay consequences exhibited that exosomes at concentrations of 50-400 ng/ml could boost the proliferation of the LSCs in vitro. Also, treatment caused the up-regulation of the expression of beta-catenin, Wnt, p38 MAPK, and ERK, partially or efficiently, in the LSCs. Conclusions: We suggested that the exosome is capable of provoking the proliferation of the LSCs by up-regulating the proliferation involved pathways. © 2023 Turkish Society of Nephrology. All rights reserved.
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