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Design, Optimization and Characterization of a Novel Antibacterial Chitosan-Based Hydrogel Dressing for Promoting Blood Coagulation and Full-Thickness Wound Healing: A Biochemical and Biophysical Study Publisher Pubmed



Mirhaji SS1 ; Soleimanpour M1 ; Derakhshankhah H2 ; Jafari S2 ; Mamashli F1 ; Rooki M3 ; Karimi MR4 ; Nedaei H1 ; Pirhaghi M1 ; Motasadizadeh H5 ; Ghasemi A1 ; Nezamtaheri MS1 ; Saadatpour F6 ; Goliaei B1 Show All Authors
Authors
  1. Mirhaji SS1
  2. Soleimanpour M1
  3. Derakhshankhah H2
  4. Jafari S2
  5. Mamashli F1
  6. Rooki M3
  7. Karimi MR4
  8. Nedaei H1
  9. Pirhaghi M1
  10. Motasadizadeh H5
  11. Ghasemi A1
  12. Nezamtaheri MS1
  13. Saadatpour F6
  14. Goliaei B1
  15. Delattre C7, 8
  16. Saboury AA1
Show Affiliations
Authors Affiliations
  1. 1. Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
  2. 2. Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
  3. 3. Department of Life Science Engineering, Faculty of New Science and Technologies, University of Tehran, Iran
  4. 4. School of Chemistry, College of Science, University of Tehran, Tehran, Iran
  5. 5. Department of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. School of Biology, College of Science, University of Tehran, Tehran, Iran
  7. 7. Institut Universitaire de France (IUF), 1 rue Descartes, Paris, 75005, France
  8. 8. Universite Clermont Auvergne, CNRS, Clermont Auvergne INP, Institut Pascal, Clermont-Ferrand, F-63000, France

Source: International Journal of Biological Macromolecules Published:2023


Abstract

The use of hydrogel dressings has become increasingly popular as a scaffold for skin tissue engineering. Herein, we have developed an innovative wound dressing using chitosan, fibrinogen, nisin, and EDTA as an effective antibacterial scaffold for wound treatment. The structural and functional characteristics of the hydrogel, including morphology, mechanical strength, drug encapsulation and release, swelling behaviors, blood coagulation, cytotoxicity, and antibacterial activity, were studied. Spectroscopic studies indicated that the attachment of chitosan to fibrinogen is associated with minimal change in its secondary structure; subsequently, at higher temperatures, it is expected to preserve fibrinogen's conformational stability. Mechanical and blood coagulation analyses indicated that the incorporation of fibrinogen into the hydrogel resulted in accelerated clotting and enhanced mechanical properties. Our cell studies showed biocompatibility and non-toxicity of the hydrogel along with the promotion of cell migration. In addition, the prepared hydrogel indicated an antibacterial behavior against both Gram-positive and Gram-negative bacteria. Interestingly, the in vivo data revealed enhanced tissue regeneration and recovery within 17 days in the studied animals. Taken together, the results obtained from in vitro and histological assessments indicate that this innovatively designed hydrogel shows good potential as a candidate for wound healing. © 2023 Elsevier B.V.
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