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Cell-Loaded Genipin Cross-Linked Collagen/Gelatin Skin Substitute Adorned With Zinc-Doped Bioactive Glass-Ceramic for Cutaneous Wound Regeneration Publisher Pubmed



Sharifi E1, 2 ; Yousefiasl S3 ; Laderian N4 ; Rabiee N5 ; Makvandi P6 ; Pourmotabed S7 ; Ashrafizadeh M8, 9 ; Familsattarian F10 ; Fang W11
Authors
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Authors Affiliations
  1. 1. Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Science, Shahrekord, 8815713471, Iran
  2. 2. Cancer Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
  3. 3. Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, 1417614411, Iran
  4. 4. School of Medicine, Shahrekord University of Medical Science, Shahrekord, 8815713471, Iran
  5. 5. School of Engineering, Macquarie University, Sydney, 2109, NSW, Australia
  6. 6. School of Engineering, Institute for Bioengineering, The University of Edinburgh, Edinburgh, EH9 3JL, United Kingdom
  7. 7. Department of Emergency Medicine, School of Medicine, Hamadan University of Medical Sciences, Hamadan, 6517838736, Iran
  8. 8. Department of General Surgery and Integrated Chinese and Western Medicine, Institute of Precision Diagnosis and Treatment of Gastrointestinal Tumors, Carson International Cancer Center, Shenzhen University General Hospital, Shenzhen University, Guangdong, Shenzhen, 518060, China
  9. 9. Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, 200032, China
  10. 10. Department of Materials Engineering, Bu-Ali Sina University, P.O.B: 65178-38695, Hamedan, Iran
  11. 11. Department of Laser and Aesthetic Medicine, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China

Source: International Journal of Biological Macromolecules Published:2023


Abstract

An optimal tissue-engineered dermal substitute should possess biocompatibility and cell adhesion conduction to facilitate fibroblast and keratinocyte infiltration and proliferation, as well as angiogenesis potential to escalate wound healing. Zinc was doped to bioactive glass-ceramic (Zn-BGC) to promote biocompatibility and angiogenesis properties. Zn-BGC was then incorporated into a collagen (Col) and gelatin (Gel) porous scaffold. The bioactive porous bionanocomposite exhibited biocompatibility along with improved cell attachment and proliferation. Scaffolds including Col-Gel/Zn-BGC with or without mouse embryonic fibroblasts were applied on full-thickness skin wounds on the BALB/c mice to assess their wound healing potential in vivo. The results indicated that the biodegradation rate of the Col-Gel/Zn-BGC nanocomposites was comparable to the rate of skin tissue regeneration in vivo. Macroscopic wound healing results showed that Col-Gel/Zn-BGC loaded with mouse embryonic fibroblast possesses the smallest wound size, indicating the fastest healing process. Histopathological evaluations displayed that the optimal wound regeneration was observed in Col-Gel/Zn-BGC nanocomposites loaded with mouse embryonic fibroblasts indicated by epithelialization and angiogenesis; besides the number of fibroblasts and hair follicles was increased. The bioactive nanocomposite scaffold of Col-Gel containing Zn-BGC nanoparticles loaded with mouse embryonic fibroblasts can be employed as a desirable skin substitute to ameliorate cutaneous wound regeneration. © 2023
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