The Comparison Between the Osteogenic Differentiation Potential of Clay-Polya-Crylonitrile Nanocomposite Scaffold and Graphene-Polyacrylonitrile Scaffold in Human Mesenchymal Stem Cells

The Comparison Between the Osteogenic Differentiation Potential of Clay-Polya-Crylonitrile Nanocomposite Scaffold and Graphene-Polyacrylonitrile Scaffold in Human Mesenchymal Stem Cells Publisher

Mirakabad FST¹ ; Hosseinzadeh S² ; Abbaszadeh HA³ ; Khoramgah MS¹ ; Ghanbarian H¹ ; Ranjbari J¹ ; Kazemi B¹

Show Affiliations

1. Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Science, Tehran, Iran
2. Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3. Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Nano Biomedicine and Engineering Published:2019

Abstract

Nowadays, bone repair by means of stem cells potential is considered as a new approach in regenerative medicine. Adipose-derived mesenchymal stem cells (AD-MSCs) have been investigated as a plentiful cell source with the ability of osteogenic differentiation which can play an important role in bone tissue engineering applications. Discovering proper elements in combination of scaffolds structure to stimulate osteogenesis in adipose-derived stem cells is one of the major concerns in this issue. Porous polymeric scaffolds such as polyacrylonitrile (PAN) and susceptible nanoparticles have attracted a lot of attention recently due to biodegradability and differentiation potential respectively. In the present study, clay-PAN nanocomposite (CPN) and graphene-PAN scaffold have been electrospuned separately and evaluated from the point of the osteogenic potential in AD-MSCs. The objective of this study was to determine the effect of clay and graphene nanoparticles with PAN nanofibers on the fate of viability and osteogenesis of AD-MSCs. First, isolated mesenchymal cells were characterized by flow cytometry. After cell culture on the surface of scaffolds MTT assay, scanning electron microscope (SEM) and DAPI staining were done. The scaffolds were characterized and osteogenic differentiation potential of AD-MSCs has been investigated. The results have indicated that alkaline phosphatase (ALP) activity, calcium content and collagen expression of cells which cultured on clay-PAN nanofibers were higher than cells which cultured on graphene-PAN scaffold. Taken together, these results suggest that porous nanofiber clay-PAN scaffold can enhance the osteogenic differentiation of AD-MSCs, and can be used as a new biodegradable scaffold for bone tissue engineering applications. © 2019 Fatemeh Sadat Tabatabaei Mirakabad, Simzar Hosseinzadeh, Hojjat Allah Abbaszadeh, Maryam Sadat Khoramgah, Hossein Ghanbarian, Javad Ranjbari, and Bahram Kazemi.

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Experts (# of related papers)

Seyed Mahdi Rezayat Sorkhabadi (1)

Style	Citing Format
MLA	Mirakabad FST, et al.. "The Comparison Between the Osteogenic Differentiation Potential of Clay-Polya-Crylonitrile Nanocomposite Scaffold and Graphene-Polyacrylonitrile Scaffold in Human Mesenchymal Stem Cells." Nano Biomedicine and Engineering, vol. 11, no. 3, 2019, pp. 238-253.
APA	Mirakabad FST, Hosseinzadeh S, Abbaszadeh HA, Khoramgah MS, Ghanbarian H, Ranjbari J, Kazemi B (2019). The Comparison Between the Osteogenic Differentiation Potential of Clay-Polya-Crylonitrile Nanocomposite Scaffold and Graphene-Polyacrylonitrile Scaffold in Human Mesenchymal Stem Cells. Nano Biomedicine and Engineering, 11(3), 238-253.
Chicago	Mirakabad FST, Hosseinzadeh S, Abbaszadeh HA, Khoramgah MS, Ghanbarian H, Ranjbari J, Kazemi B. "The Comparison Between the Osteogenic Differentiation Potential of Clay-Polya-Crylonitrile Nanocomposite Scaffold and Graphene-Polyacrylonitrile Scaffold in Human Mesenchymal Stem Cells." Nano Biomedicine and Engineering 11, no. 3 (2019): 238-253.
Harvard	Mirakabad FST et al. (2019) 'The Comparison Between the Osteogenic Differentiation Potential of Clay-Polya-Crylonitrile Nanocomposite Scaffold and Graphene-Polyacrylonitrile Scaffold in Human Mesenchymal Stem Cells', Nano Biomedicine and Engineering, 11(3), pp. 238-253.
Vancouver	Mirakabad FST, Hosseinzadeh S, Abbaszadeh HA, Khoramgah MS, Ghanbarian H, Ranjbari J, et al.. The Comparison Between the Osteogenic Differentiation Potential of Clay-Polya-Crylonitrile Nanocomposite Scaffold and Graphene-Polyacrylonitrile Scaffold in Human Mesenchymal Stem Cells. Nano Biomedicine and Engineering. 2019;11(3):238-253.
BibTex	@article{ author = {Mirakabad FST and Hosseinzadeh S and Abbaszadeh HA and Khoramgah MS and Ghanbarian H and Ranjbari J and Kazemi B}, title = {The Comparison Between the Osteogenic Differentiation Potential of Clay-Polya-Crylonitrile Nanocomposite Scaffold and Graphene-Polyacrylonitrile Scaffold in Human Mesenchymal Stem Cells}, journal = {Nano Biomedicine and Engineering}, volume = {11}, number = {3}, pages = {238-253}, year = {2019} }
RIS	TY - JOUR AU - Mirakabad FST AU - Hosseinzadeh S AU - Abbaszadeh HA AU - Khoramgah MS AU - Ghanbarian H AU - Ranjbari J AU - Kazemi B TI - The Comparison Between the Osteogenic Differentiation Potential of Clay-Polya-Crylonitrile Nanocomposite Scaffold and Graphene-Polyacrylonitrile Scaffold in Human Mesenchymal Stem Cells JO - Nano Biomedicine and Engineering VL - 11 IS - 3 SP - 238 EP - 253 PY - 2019 ER -

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