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Poly (Glycerol Sebacate) and Polyhydroxybutyrate Electrospun Nanocomposite Facilitates Osteogenic Differentiation of Mesenchymal Stem Cells Publisher



Abazari MF1 ; Zare Karizi S2 ; Samadian H3 ; Nasiri N4 ; Askari H5 ; Asghari M6 ; Frootan F7 ; Bakhtiari H8 ; Mahboudi H9 ; Mansouri V10
Authors
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Authors Affiliations
  1. 1. Research Center for Clinical Virology, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Department of biology, Varamin Pishva Branch, Islamic Azad University, Pishva, Varamin, Iran
  3. 3. Nano Drug Delivery Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
  4. 4. Institute of Molecular Biology, Vrije Universiteit Brussel, Brussels, Belgium
  5. 5. Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
  6. 6. Department of Molecular Biotechnology, Cell Science Research Center, Royan Institute of Biotechnology, ACECR, Isfahan, Iran
  7. 7. Institute of Agricultural Biotechnology, National Institute of Genetic Engineering & Biotechnology (NIGEB), Tehran, Iran
  8. 8. Department of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
  9. 9. Department of Biotechnology, School of Pharmacy, Alborz University of Medical Sciences, Karaj, Iran
  10. 10. Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Journal of Drug Delivery Science and Technology Published:2021


Abstract

Bone tissue engineering is a rapidly growing approach for repairing bone lesions, which needs scaffolds that meet biomechanical and bio-structural requirements of target tissues. The aim of the present study is to demonstrate the potential application of fabricated poly (glycerol sebacate) and Polyhydroxybutyrate (PGS-PHB) nanofibrous scaffold by electrospinning, as a potential bone implant. Herein, after morphological study by SEM, the biological behavior and toxicity of the constructed scaffold were evaluated by cell attachment, protein adsorption and MTT assay. Then the supportive potential of the scaffold upon osteogenic differentiation process was investigated by the culture of adipose tissue-derived mesenchymal stem cells (ADSCs). The results showed that the PGS-PHB scaffold was nanofibrous, with a uniform surface and good porosity. The protein adsorption capacity of the scaffold was significantly higher than tissue culture plate (TCP) as a control group. The initial cell attachment in the PGS-PHB scaffold and TCP was not significantly different. Finally, the osteogenic differentiation potential of the ADSCs cultured on the PGS-PHB scaffold and TCP were evaluated by assessment of alkaline phosphatase (ALP) activity, calcium content and bone-related gene expression. The results revealed that the ALP activity, calcium producing and expression level of bone-related genes in the cultured cells in the PGS-PHB scaffold group was significantly higher than cultured cells in the control group. Based on the obtained results, the constructed PGS-PHB scaffold has promising potential for use in bone tissue engineering applications. © 2021
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